ATP5A1 Polyklonaler Antikörper

ATP5A1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(165)

Rezensionen (3)

Kat-Nr. : 14676-1-AP

Synonyme

ATP synthase F1 subunit alpha, ATP5A, ATP5A 1

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  WB analysis of various samples using 14676-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with PK30006 (OXPHOS Cocktail (Human recommended)) at dilution of 1:4000 incubated at room temperature for 1 hours.

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  WB analysis using 14676-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14676-1-AP (ATP5A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14676-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14676-1-AP (ATP5A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14676-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14676-1-AP (ATP5A1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14676-1-AP

  IP result of anti-ATP5A1 (IP:14676-1-AP, 4ug; Detection:14676-1-AP 1:15000) with HeLa cells lysate 1750 ug.

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  IHC staining of human colon using 14676-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14676-1-AP (ATP5A1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 14676-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14676-1-AP (ATP5A1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse brain using 14676-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14676-1-AP (ATP5A1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 14676-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14676-1-AP (ATP5A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 14676-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14676-1-AP (ATP5A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 14676-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14676-1-AP (ATP5A1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 14676-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14676-1-AP (ATP5A1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 14676-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14676-1-AP (ATP5A1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14676-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATP5A1 antibody (14676-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 14676-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ATP5A1 antibody (14676-1-AP) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Maushirngewebe, Mausherzgewebe, Mauslebergewebe, Rattenhirngewebe, Rattenherzgewebe, Rattenlebergewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Kolongewebe, humanes Nierengewebe, humanes Lebergewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 147 publications below
• IHC: See 5 publications below
• IF: See 21 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below

Produktinformation

14676-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA ATP5A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, hamster, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP5A1 fusion protein Ag6385
• Vollständiger Name: ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC064562
• Gene symbol: ATP5A1
• Gene ID (NCBI): 498
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The ATP5A1 gene encodes the α subunit of mitochondrial ATP synthase which produces ATP from ADP in the presence of a proton gradient across the membrane. The mitochondrial ATP synthase, also known as Complex V or F1F0 ATP synthase, is a multi-subunit enzyme complex consisting of two functional domains, the F1-containing the catalytic core and the Fo-containing the membrane proton channel. F0 domain has 10 subunits: a,b, c, d, e, f, g, OSCP, A6L, and F6. F1 is composed of subunits α, β, γ, δ, ε, and a loosely attached inhibitor protein IF1. Recently defect in ATP5A1 has been linked to the fatal neonatal mitochondrial encephalopathy. ATP5A1 is localized in the mitochondria and anti-ATP5A1 can be used as the loading control for mitochondrial or Complex V proteins. This antibody recognizes the endogenous ATP5A1 protein in lysates from various cell lines and tissues. The predicted MW of ATP5A1 is 60 kDa, while it undergoes the transit peptide cleavage to become a mature form around 50-55 kDa. Several isoforms of ATP5A1 exist due to the alternative splicing.

Publikationen

Species	Application	Title
mouse	WB	Cell Res; Disruption of ER ion homeostasis maintained by an ER anion channel CLCC1 contributes to ALS-like pathologies; Authors - Liang Guo
mouse	WB	Mol Cell; RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages; Authors - Teodorus Theo Susanto
human	WB	Mol Cell; Global mitochondrial protein import proteomics reveal distinct regulation by translation and translocation machinery; Authors - Jasmin Adriana Schafer
mouse	WB	Nat Chem Biol; Small molecule agonist of mitochondrial fusion repairs mitochondrial dysfunction; Authors - Yingjie Guo
mouse	WB	Cell Rep Med; Targeting mitochondrial complex I of CD177+ neutrophils alleviates lung ischemia-reperfusion injury; Authors - Junqi Wu
mouse	WB	Sci Adv; Mitochondrial proteostasis stress in muscle drives a long-range protective response to alleviate dietary obesity independently of ATF4.; Authors - Qiqi Guo

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Edward (Verified Customer) (11-15-2022)

Used with 12 ug protein lysate ATP5A1 works well, both with ECL detection and fluorescent secondary antibodies it gives a clear band for K562 and KCL22, though no band was apparent in THP-1 (this could be cell line specific).

• Applications: Western Blot
• Primary Antibody Dilution: 1:8000
• Cell Tissue Type: K562, KCL22 and THP-1

FH

XB (Verified Customer) (10-29-2020)

It probes ATP5A1 clearly with a right MW 55 kD.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: human IPSC-derived neurons

ATP5A1 Antibody Western Blot validation (1:2000 dilution) in human IPSC-derived neurons (Cat no:14676-1-AP)

FH

Siting (Verified Customer) (09-04-2020)

Good antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart

ATP5A1 Antibody Western Blot validation (1:1000 dilution) in Mouse heart (Cat no:14676-1-AP)