ATP5A1 Monoklonaler Antikörper

ATP5A1 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Affe, human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B10H3

Kat-Nr. : CL488-66037

Synonyme

ATP5A, ATP5A1, ATP5AL2, ATPM, Cell and organelle markers, hATP1, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker, MOM2, OMR, ORM



Geprüfte Anwendungen

Erfolgreiche Detektion in IFHepG2-Zellen, HeLa-Zellen
Erfolgreiche Detektion in FCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66037 bindet in IF, FC (Intra) ATP5A1 und zeigt Reaktivität mit Affe, human, Maus, Ratten

Getestete Reaktivität Affe, human, Maus, Ratte
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen ATP5A1 fusion protein Ag8119
Vollständiger Name ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
Berechnetes Molekulargewicht 60 kDa
GenBank-ZugangsnummerBC064562
Gene symbol ATP5A1
Gene ID (NCBI) 498
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The ATP5A1 gene encodes the α subunit of mitochondrial ATP synthase which produces ATP from ADP in the presence of a proton gradient across the membrane. The mitochondrial ATP synthase, also known as Complex V or F1F0 ATP synthase, is a multi-subunit enzyme complex consisting of two functional domains, the F1-containing the catalytic core and the Fo- containing the membrane proton channel. F0 domain has 10 subunits: a, b, c, d, e, f, g, OSCP, A6L, and F6. F1 is composed of subunits α, β, γ, δ, ε, and a loosely attached inhibitor protein IF1. Recently defect in ATP5A1 has been linked to the fatal neonatal mitochondrial encephalopathy. ATP5A1 is localized in the mitochondria and anti-ATP5A1 can be used as the loading control for mitochondrial or Complex V proteins. This antibody recognizes the endogenous ATP5A1 protein in lysates from various cell lines and tissues. The predicted MW of ATP5A1 is 60 kDa, while it undergoes the transit peptide cleavage to become a mature form around 50-55 kDa. Several isoforms of ATP5A1 exist due to the alternative splicing.

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 ATP5A1 antibody CL488-66037Protokoll herunterladen
Standard-Protokolle
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