ATP5F1 Polyklonaler Antikörper

ATP5F1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(28)

Rezensionen (2)

Kat-Nr. : 15999-1-AP

Synonyme

ATP5PB, ATP5F 1, ATP synthase subunit b, ATP synthase proton-transporting mitochondrial F(0) complex subunit B1, ATP synthase peripheral stalk-membrane subunit b

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  WB analysis using 15999-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15999-1-AP (ATP5F1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 15999-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15999-1-AP (ATP5F1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 15999-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15999-1-AP (ATP5F1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 15999-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15999-1-AP (ATP5F1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 15999-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 15999-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 15999-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 15999-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 15999-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 15999-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 15999-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 15999-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 15999-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 15999-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 15999-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 15999-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 15999-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ATP5F1 antibody (15999-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  FC experiment of HeLa using 15999-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATP5F1 (15999-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Mauslebergewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Hirngewebe, humanes Herzgewebe, humanes Nierengewebe, humanes Hautgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 25 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

15999-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA ATP5F1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP5F1 fusion protein Ag8571
• Vollständiger Name: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1
• Berechnetes Molekulargewicht: 256 aa, 29 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC005366
• Gene symbol: ATP5F1
• Gene ID (NCBI): 515
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP5F1(ATP synthase subunit b) belongs to the eukaryotic ATPase B chain family. The ATP5F1 gene encodes subunit B of the mitochondrial ATP synthase Fo unit, which contains 214-amino acid with a 42-amino acid import signal(PMID:1831354). Mitochondrial membrane ATP synthase(F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain.

Publikationen

Species	Application	Title
human	IF	Adv Mater; Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration; Authors - Zhuo Li
rat	WB	Proc Natl Acad Sci U S A; Evaluation of intramitochondrial ATP levels identifies G0/G1 switch gene 2 as a positive regulator of oxidative phosphorylation.; Authors - Hidetaka Kioka
mouse	WB,IHC	Part Fibre Toxicol; Transcriptomics of single dose and repeated carbon black and ozone inhalation co-exposure highlight progressive pulmonary mitochondrial dysfunction.; Authors - Quincy A Hathaway
human	WB	Free Radic Biol Med; Mitochondrial and glucose metabolic dysfunctions in granulosa cells induce impaired oocytes of polycystic ovary syndrome through Sirtuin 3.; Authors - Qing Zhang
mouse	WB	J Neurosci; A Mutation in Hnrnph1 That Decreases Methamphetamine-Induced Reinforcement, Reward, and Dopamine Release and Increases Synaptosomal hnRNP H and Mitochondrial Proteins.; Authors - Qiu T Ruan
rat	WB	Cell Death Dis; Calcium sensing receptor protects high glucose-induced energy metabolism disorder via blocking gp78-ubiquitin proteasome pathway.; Authors - Yuehong Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

siting (Verified Customer) (07-13-2020)

very good signal

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse heart cells

ATP5F1 Antibody Western Blot, validation (1:1000 dilution) in mouse heart cells (Cat no:15999-1-AP)

FH

Yan (Verified Customer) (01-28-2020)

It worked very well with mouse heart tissue for western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue extract