ATP6V1B2 Polyklonaler Antikörper

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• KD/KO Validated

ATP6V1B2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 15097-1-AP

Synonyme

ATP6B1B2, ATP6B2, ATP6V1B2, HO57, V ATPase subunit B 2, VATB, Vma2, VPP3

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Galerie der Validierungsdaten

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  WB analysis of mouse heart using 15097-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15097-1-AP (ATP6V1B2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15097-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15097-1-AP (ATP6V1B2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15097-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15097-1-AP (ATP6V1B2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15097-1-AP

  WB result of ATP6V1B2 antibody (15097-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP6V1B2 transfected HeLa cells.

• 

  WB analysis of mouse skeletal muscle using 15097-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15097-1-AP (ATP6V1B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15097-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15097-1-AP (ATP6V1B2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15097-1-AP

  IP Result of anti-ATP6V1B2 (IP:15097-1-AP, 4ug; Detection:15097-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human colon cancer using 15097-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15097-1-AP (ATP6V1B2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 15097-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15097-1-AP (ATP6V1B2 antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, K-562-Zellen, Mausherzgewebe, Maushirngewebe, Maus-Skelettmuskelgewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:40000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IP: See 1 publications below

Produktinformation

15097-1-AP bindet in WB, IP, IHC, ELISA ATP6V1B2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP6V1B2 fusion protein Ag7174
• Vollständiger Name: ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 56-58 kDa
• GenBank-Zugangsnummer: BC003100
• Gene symbol: ATP6V1B2
• Gene ID (NCBI): 526
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP6V1B2(V-type proton ATPase subunit B, brain isoform) is also named as ATP6B2, VPP3,HO57. It belongs to the ATPase alpha/beta chains family.V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.The quasi-ubiquitous ATP6V1B2 is one of 2 isoforms of ATP6V1 and is expressed in most cell types, where it plays a major role in organelle acidification(PMID:18667600).

Publikationen

Species	Application	Title
human	WB	Nat Microbiol; The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry.; Authors - Tomas Doyle
human	WB,IP	Autophagy; KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity.; Authors - Guokai Yan
mouse	WB	Cell Mol Life Sci; The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.; Authors - Enrico Castroflorio
pig	WB	Microorganisms; Exopolysaccharides from Bifidobacterium animalis Ameliorate Escherichia coli-Induced IPEC-J2 Cell Damage via Inhibiting Apoptosis and Restoring Autophagy.; Authors - Lanxin Yuan
human	WB	J Cancer; Upregulation of V-ATPase by STAT3 Activation Promotes Anoikis Resistance and Tumor Metastasis.; Authors - Funmilayo O Adeshakin
human	WB	Vet Res; Butyrate-mediated autophagy inhibition limits cytosolic Salmonella Infantis replication in the colon of pigs treated with a mixture of Lactobacillus and Bacillus.; Authors - Bingxin Chu