ATP6V1G1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ATP6V1G1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Kat-Nr. : 16143-1-AP

Synonyme

V-ATPase 13 kDa subunit 1, V ATPase subunit G 1, ATP6J, ATP6GL, ATP6G1

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  WB analysis of Jurkat using 16143-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 16143-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 16143-1-AP

  IP result of anti-ATP6V1G1 (IP:16143-1-AP, 4ug; Detection:16143-1-AP 1:500) with Jurkat cells lysate 3440 ug.

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  IHC staining of human kidney using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HEK-293 using 16143-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, humanes Nierengewebe
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Hirngewebe, humanes Herzgewebe, humanes Lebergewebe, humanes Eierstockgewebe, humanes Plazenta-Gewebe, humanes Hautgewebe, humanes Milzgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

16143-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA ATP6V1G1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP6V1G1 fusion protein Ag9217
• Vollständiger Name: ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G1
• Berechnetes Molekulargewicht: 118 aa, 14 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC008452
• Gene symbol: ATP6V1G1
• Gene ID (NCBI): 9550
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Traffic; Fast and cloning-free CRISPR/Cas9-mediated genomic editing in mammalian cells.; Authors - Paul T Manna
rat	WB	J Cell Physiol; A pH-sensitive luminal His-cluster promotes interaction of PAM with V-ATPase along the secretory and endocytic pathways of peptidergic cells.; Authors - Vishwanatha K Rao
Human	WB	PLoS One; Acidification of endothelial Weibel-Palade bodies is mediated by the vacuolar-type H+-ATPase.; Authors - Julian Terglane
human,mouse	WB,IHC	EBioMedicine; A GBM-like V-ATPase signature directs cell-cell tumor signaling and reprogramming via large oncosomes.; Authors - Irene Bertolini ; KD Validated
human	WB	EBioMedicine; Specific V-ATPase expression sub-classifies IDHwt lower-grade gliomas and impacts glioma growth in vivo.; Authors - Andrea Terrasi
human	WB	Autophagy; Inhibition of autophagy and induction of glioblastoma cell death by NEO214, a perillyl alcohol-rolipram conjugate; Authors - Mengting Ou