ATP6V1G3 Polyklonaler Antikörper

ATP6V1G3 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19523-1-AP

Synonyme

ATP6G3, ATP6V1G3, V ATPase 13 kDa subunit 3, V ATPase subunit G 3, Vma10

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 19523-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19523-1-AP (ATP6V1G3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 19523-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19523-1-AP (ATP6V1G3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 19523-1-AP

  IP Result of anti-ATP6V1G3 (IP:19523-1-AP, 4ug; Detection:19523-1-AP 1:800) with mouse kidney tissue lysate 4800ug.

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  IHC staining of human kidney using 19523-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19523-1-AP (ATP6V1G3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 19523-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19523-1-AP (ATP6V1G3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 19523-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19523-1-AP (ATP6V1G3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 19523-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19523-1-AP (ATP6V1G3 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Mausnierengewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

19523-1-AP bindet in WB, IP, IHC, ELISA ATP6V1G3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G3
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: NM_133326
• Gene symbol: ATP6V1G3
• Gene ID (NCBI): 127124
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP6V1G3,also named as ATP6G3, belongs to the V-ATPase G subunit family. ATP6V1G3 is a catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.