ATP7B-Specific Polyklonaler Antikörper

ATP7B-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

Kat-Nr. : 19786-1-AP

Synonyme

ATP7B, ATPase, Cu++ transporting, beta polypeptide, Copper-transporting ATPase 2, EC:7.2.2.8, WC1

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  WB analysis using 19786-1-AP

  Mouse liver and rat liver tissues were subjected to SDS PAGE followed by western blot with 19786-1-AP (ATP7B-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 19786-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19786-1-AP (ATP7B-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 19786-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19786-1-AP (ATP7B-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 19786-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19786-1-AP (ATP7B-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19786-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19786-1-AP (ATP7B-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19786-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19786-1-AP (ATP7B-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19786-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19786-1-AP (ATP7B-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse liver using 19786-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using ATP7B-Specific antibody (19786-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 19786-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATP7B-Specific antibody (19786-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mauslebergewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Produktinformation

19786-1-AP bindet in WB, IHC, IF/ICC, IF-P, ELISA ATP7B-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ATPase, Cu++ transporting, beta polypeptide
• Berechnetes Molekulargewicht: 157 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: NM_000053
• Gene symbol: ATP7B
• Gene ID (NCBI): 540
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP7B, also named as PWD, WC1 and WND, belongs to the cation transport ATPase (P-type) family and Type IB subfamily. ATP7B is involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile. ATP7B catalyzes the reaction: ATP + H2O + Cu2+(In) = ADP + phosphate + Cu2+(Out). Defects in ATP7B are the cause of Wilson disease (WD). This antibody is specific to ATP7B.

Publikationen

Species	Application	Title
mouse	WB,IHC	J Immunother Cancer; C5a/C5aR pathway blocking promoted CuS-mediated cancer therapy effect by inhibiting cuproptosis resistance; Authors - Hong Yang
mouse	WB	Adv Healthc Mater; Copper-Based Composites Nanoparticles Improve Triple-Negative Breast Cancer Treatment with Induction of Apoptosis-Cuproptosis and Immune Activation; Authors - Ning Wang
human	IHC	Comput Struct Biotechnol J; Impact of Cuproptosis-related markers on clinical status, tumor immune microenvironment and immunotherapy in colorectal cancer: A multi-omic analysis; Authors - Yanfei Shao
human	WB,IHC	Gene; Integrative analysis of the cuproptosis-related gene ATP7B in the prognosis and immune infiltration of IDH1 wild-type glioma; Authors - Kun Zhang
human	IF	Medicina (Kaunas); Curcumin Effect on Copper Transport in HepG2 Cells.; Authors - Anita Berzina
rat	WB	Ann Med; Dexmedetomidine enables copper homeostasis in cerebral ischemia/reperfusion via ferredoxin 1; Authors - Qingduo Guo