ATP8 Polyklonaler Antikörper

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• KD/KO Validated

ATP8 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(45)

Rezensionen (4)

Kat-Nr. : 26723-1-AP

Synonyme

MT-ATP8

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• 

  WB analysis of 143B.TK.P0 using 26723-1-AP

  WB result of ATP8 antibody (ATP8; 1:3000; incubated at room temperature for 1.5 hours) with 143B and 143B.TK.ρ0 cell. 143B is wild-type cell line, 143B.TK.ρ0 is mtDNA deletion cell line.

• 

  WB analysis using 26723-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26723-1-AP (ATP8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 26723-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATP8 antibody (26723-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, LNCaP-Zellen, Rattenherzgewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 44 publications below

Produktinformation

26723-1-AP bindet in WB, IF/ICC, ELISA ATP8 und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ATP synthase 8; ATPase subunit 8
• Berechnetes Molekulargewicht: 8 kDa
• Beobachtetes Molekulargewicht: 8 kDa
• GenBank-Zugangsnummer: YP_003024030
• Gene symbol: ATP8
• Gene ID (NCBI): 4509
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP8 is a subunit of F0, the functional domain of mitochondrial ATP synthase or complex V. Mutation of ATP8 has been reported as the cause of apical hypertrophic cardiomyopathy and neuropathy.

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; ALKBH7-mediated demethylation regulates mitochondrial polycistronic RNA processing.; Authors - Li-Sheng Zhang
human	WB	Mol Cell; Hepatic micropeptide modulates mitochondrial RNA processing machinery in hepatocellular carcinoma; Authors - Linyu Zhu
human	WB	Nucleic Acids Res; Selection of initiator tRNA and start codon by mammalian mitochondrial initiation factor 3 in leaderless mRNA translation; Authors - Muhoon Lee
human	WB	Sci Adv; Pathogenic mitochondrial DNA mutations inhibit melanoma metastasis; Authors - Spencer D Shelton
human	WB	Nucleic Acids Res; A coronary artery disease-associated tRNAThr mutation altered mitochondrial function, apoptosis and angiogenesis.; Authors - Zidong Jia
human	WB	Nucleic Acids Res; A deafness-associated tRNA mutation caused pleiotropic effects on the m1G37 modification, processing, stability and aminoacylation of tRNAIle and mitochondrial translation.; Authors - Feilong Meng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Emilie (Verified Customer) (09-24-2025)

Bands appeared sharp and well defined under the chosen dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Fibroblasts

FH

Manon (Verified Customer) (09-19-2025)

A distinct band is visible, but since it is at a very low molecular weight, it could be easily missed

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Fibroblasts

FH

Baptiste (Verified Customer) (05-23-2025)

Working perfectly

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Lymphoblastoid

ATP8 Antibody Western Blot validation (1:3000 dilution) in Lymphoblastoid (Cat no:26723-1-AP)

FH

Mi (Verified Customer) (02-10-2023)

This antibody works very well in human adipocytes, a strong single band around 8 kd is detected when loading 10 ug total protein.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: adipocytes