ATP8 Polyklonaler Antikörper

ATP8 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

32

Rezensionen (1)

Kat-Nr. : 26723-1-AP

Synonyme

A6L, ATP synthase protein 8, ATP8, ATPASE8, F ATPase subunit 8, MT ATP8, MTATP8

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Galerie der Validierungsdaten

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  WB analysis using 26723-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26723-1-AP (ATP8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26723-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26723-1-AP (ATP8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 26723-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 26723-1-AP (ATP8 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 26723-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATP8 antibody (26723-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, LNCaP-Zellen, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 32 publications below

Produktinformation

26723-1-AP bindet in WB, IHC, IF, ELISA ATP8 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ATP synthase 8; ATPase subunit 8
• Berechnetes Molekulargewicht: 8 kDa
• Beobachtetes Molekulargewicht: 8 kDa
• GenBank-Zugangsnummer: YP_003024030
• Gene symbol: ATP8
• Gene ID (NCBI): 4509
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP8 is a subunit of F0, the functional domain of mitochondrial ATP synthase or complex V. Mutation of ATP8 has been reported as the cause of apical hypertrophic cardiomyopathy and neuropathy.

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; ALKBH7-mediated demethylation regulates mitochondrial polycistronic RNA processing.; Authors - Li-Sheng Zhang
human	WB	Nucleic Acids Res; A coronary artery disease-associated tRNAThr mutation altered mitochondrial function, apoptosis and angiogenesis.; Authors - Zidong Jia
human	WB	Nucleic Acids Res; A deafness-associated tRNA mutation caused pleiotropic effects on the m1G37 modification, processing, stability and aminoacylation of tRNAIle and mitochondrial translation.; Authors - Feilong Meng
zebrafish	WB	Nucleic Acids Res; Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency.; Authors - Qinghai Zhang
human	WB	Nucleic Acids Res; Hypertension-associated mitochondrial DNA 4401A>G mutation caused the aberrant processing of tRNAMet, all 8 tRNAs and ND6 mRNA in the light-strand transcript.; Authors - Xiaoxu Zhao
human	WB	J Cell Biol; Parkin-independent mitophagy via Drp1-mediated outer membrane severing and inner membrane ubiquitination.; Authors - Yumiko Oshima

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mi (Verified Customer) (02-10-2023)

This antibody works very well in human adipocytes, a strong single band around 8 kd is detected when loading 10 ug total protein.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: adipocytes