ATPB Polyklonaler Antikörper

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• KD/KO Validated

ATPB Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(72)

Kat-Nr. : 17247-1-AP

Synonyme

EC:7.1.2.2, ATPSB, ATPMB, ATP5F1B, ATP5B

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  WB analysis using 17247-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17247-1-AP (ATPB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17247-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17247-1-AP (ATPB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 17247-1-AP

  IP result of anti-ATPB (IP:17247-1-AP, 3ug; Detection:17247-1-AP 1:500) with mouse heart tissue lysate 7500ug.

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  IHC staining of human colon using 17247-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 17247-1-AP (ATPB antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 17247-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATPB antibody (17247-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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  IF Staining of HeLa using 17247-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATPB antibody (17247-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, HepG2-Zellen, HT-29-Zellen, Maushirngewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: human colon; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:300-1:1200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 66 publications below
• IHC: See 3 publications below
• IF: See 5 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below

Produktinformation

17247-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA ATPB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATPB fusion protein Ag11177
• Vollständiger Name: ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
• Berechnetes Molekulargewicht: 529 aa, 57 kDa
• Beobachtetes Molekulargewicht: 45-57 kDa
• GenBank-Zugangsnummer: BC016512
• Gene symbol: ATPB
• Gene ID (NCBI): 506
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP5B, also named as ATPMB and ATPSB, belongs to the ATPase alpha/beta chains family. It is a high affinity HDL receptor for apolipoprotein A1. ATP5B is a subunit of mitochondrial ATP synthase (F1F0 ATP synthase or Complex V ). ATP5B has a calculated molecular mass of 57 kDa and ~5 kDa transit peptide can be removed in the mature form.

Publikationen

Species	Application	Title
human	WB	Nature; DNA of neutrophil extracellular traps promotes cancer metastasis via CCDC25.; Authors - Linbin Yang
mouse	WB,IF	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
mouse	WB	Sci Adv; Proteomimetic polymer blocks mitochondrial damage, rescues Huntington's neurons, and slows onset of neuropathology in vivo; Authors - Wonmin Choi
human	WB	Nat Commun; Small-molecule suppression of calpastatin degradation reduces neuropathology in models of Huntington's disease.; Authors - Di Hu
mouse	WB	Nat Commun; ATAD3A oligomerization promotes neuropathology and cognitive deficits in Alzheimer's disease models.; Authors - Yuanyuan Zhao
human	WB	Brain; Ligand-free mitochondria-localized mutant AR-induced cytotoxicity in spinal bulbar muscular atrophy.; Authors - Xia Feng