Alpha-1-Antitrypsin Rekombinanter Antikörper

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• KD/KO Validated

Alpha-1-Antitrypsin Rekombinant Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

230109A8

Kat-Nr. : 82918-1-RR

Synonyme

a1-antitrypsin, Serpin A1, SERPINA1, AAT, A1AT

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  WB analysis using 82918-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82918-1-RR (Alpha-1-Antitrypsin antibody) at dilution of 1:32000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 82918-1-RR

  WB result of Alpha-1-Antitrypsin antibody (82918-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alpha-1-Antitrypsin transfected HepG2 cells.

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  IHC staining of human colon using 82918-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82918-1-RR (Alpha-1-Antitrypsin antibody) at dilution of 1:6400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 82918-1-RR

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 82918-1-RR (Alpha-1-Antitrypsin antibody) at dilution of 1:6400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HepG2 using 82918-1-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Alpha-1-Antitrypsin Recombinant antibody (82918-1-RR, Clone:230109A8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82918-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82918-1-RR against Human Alpha-1-Antitrypsin were performed. The affinity constant is 0.719 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe, HepG2-Zellen, humanes Herzgewebe, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:3200-1:12800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82918-1-RR bindet in WB, IHC, FC (Intra), ELISA Alpha-1-Antitrypsin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Alpha-1-Antitrypsin fusion protein Ag9369
• Vollständiger Name: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
• Berechnetes Molekulargewicht: 418 aa, 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC015642
• Gene symbol: Alpha 1-Antitrypsin
• Gene ID (NCBI): 5265
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SERPINA1 is the gene for a protein called alpha-1-antitrypsin (AAT), which is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. AAT is a glycoprotein synthesized primarily by hepatocytes, with smaller amountssynthesized by intestinal epithelial cells, neutrophils, pulmonary alveolar cells and macrophages. AAT is the most abundant, endogenous serine protease inhibitor in blood circulation and it has been implicated in regulating vital fluid phase biological events such as blood coagulation, fibrinolysis, complement activation, apoptosis, reproduction, tumor progression and inflammatory response. The primary function of AAT is thought to be the inactivation of neutrophil elastase and other endogenous serine proteases. Defects in SERPINA1 can cause emphysema or liver disease.