Alpha Actinin Polyklonaler Antikörper

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• KD/KO Validated

Alpha Actinin Polyklonal Antikörper für IF-P, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF-P, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-11313

Synonyme

ACTN1, Alpha actinin 1, actin, actinin, alpha 1, ACTN 1

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  IF Staining of rat heart using CL488-11313

  Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using CoraLite® Plus 488 Alpha Actinin antibody (CL488-11313) at dilution of 1:200.

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  IF Staining of rat heart using CL488-11313

  Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using CoraLite® Plus 488 Alpha Actinin antibody (CL488-11313) at dilution of 1:200.

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  FC experiment of A431 using CL488-11313

  1X10^6 A431 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Alpha Actinin (CL488-11313) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of H9C2 using CL488-11313

  1x10^6 H9C2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated Alpha Actinin Polyclonal antibody (CL488-11313)(red), or 0.4 ug CoraLite® Plus 488 Rabbit IgG Isotype Control RecAb (CL488-98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Rattenherzgewebe
• Erfolgreiche Detektion in FC (Intra): A431-Zellen, H9C2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-11313 bindet in IF-P, FC (Intra) Alpha Actinin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Alpha Actinin fusion protein Ag1859
• Vollständiger Name: actinin, alpha 1
• Berechnetes Molekulargewicht: 103 kDa
• Beobachtetes Molekulargewicht: 100-105 kDa
• GenBank-Zugangsnummer: BC015766
• Gene symbol: Alpha Actinin
• Gene ID (NCBI): 87
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.