Alpha Tubulin Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

Alpha Tubulin Rekombinant Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1G7

Kat-Nr. : CL594-80762

Synonyme

TUBA1B, Tubulin alpha 1B chain, 1G7, Alpha-tubulin ubiquitous, EC:3.6.5.-

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  IF Staining of human adipose-derived mesenchymal stem cells using CL594-80762

  IF in human adipose-derived mesenchymal stem cells undergoing adipogenesis. Sample was stained for neutral fat droplets (orange), alpha-tubulin using CoraLite 594-conjugated Alpha Tubulin Recombinant Antibody (CL594-80762, white), adiponectin using Adiponectin Mouse Monoclonal Antibody (66239-1-Ig) and Multi-rAb CoraLite Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM005, green). Nuclei shown in red. ( Image credit: Shuntaro Yamada, PhD, University of Bergen)

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  IF Staining of human adipose-derived mesenchymal stem cells using CL594-80762

  IF in human adipose-derived mesenchymal stem cells undergoing adipogenesis. Sample was stained for neutral fat droplets (green), alpha-tubulin using CoraLite 594-conjugated Alpha Tubulin Recombinant Antibody (CL594-80762, red), and adiponectin using Adiponectin Mouse Monoclonal Antibody (66239-1-Ig) and Multi-rAb CoraLite Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM005, yellow). Nuclei shown in blue. (Image credit: Shuntaro Yamada, PhD, University of Bergen)

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  IF Staining of human adipose-derived mesenchymal stem cells using CL594-80762

  IF in human adipose-derived mesenchymal stem cells undergoing adipogenesis. Sample was stained for neutral fat droplets (green), alpha-tubulin using CoraLite 594-conjugated Alpha Tubulin Recombinant Antibody (CL594-80762, red), and adiponectin using Adiponectin Mouse Monoclonal Antibody (66239-1-Ig) and Multi-rAb CoraLite Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM005, yellow). Nuclei shown in blue. ( Image credit: Shuntaro Yamada, PhD, University of Bergen)

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  IF Staining of human adipose-derived mesenchymal stem cells using CL594-80762

  IF in human adipose-derived mesenchymal stem cells undergoing adipogenesis. Sample was stained for neutral fat droplets (green), alpha-tubulin using CoraLite 594-conjugated Alpha Tubulin Recombinant Antibody (CL594-80762, white), and adiponectin using Adiponectin Mouse Monoclonal Antibody (66239-1-Ig) and Multi-rAb CoraLite Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM005, red). Nuclei shown in blue. (Image credit: Shuntaro Yamada, PhD, University of Bergen)

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  IF Staining of HepG2 using CL594-80762

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 Alpha Tubulin antibody (CL594-80762, Clone: 1G7 ) at dilution of 1:200.

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  FC experiment of HepG2 using CL594-80762

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated Alpha Tubulin Recombinant antibody (CL594-80762, Clone:1G7)(red), or 0.4 ug CoraLite®594-conjugated Rabbit IgG control Rabbit PolyAb (CL594-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, human adipose-derived mesenchymal stem cells
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-80762 bindet in IF/ICC, FC (Intra) Alpha Tubulin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Alpha Tubulin fusion protein Ag18034
• Vollständiger Name: tubulin, alpha 1b
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC009314
• Gene symbol: Alpha Tubulin
• Gene ID (NCBI): 10376
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha tubulin is useful for scientists across fields as an internal control due to its high, ubiquitous expression pattern.