Annexin A1 Monoklonaler Antikörper

Annexin A1 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1E1B7

Kat-Nr. : CL594-66344

Synonyme

Annexin 1, annexin A1, Annexin I, ANX1, ANXA1, Calpactin 2, Calpactin II, Chromobindin 9, Lipocortin I, LPC1, p35

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Galerie der Validierungsdaten

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  IF Staining of A549 using CL594-66344

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite®594 Annexin A1 antibody (CL594-66344, Clone: 1E1B7 ) at dilution of 1:200.

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  FC experiment of K-562 using CL594-66344

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Annexin A1 (CL594-66344, Clone:1E1B7) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of Jurkat using CL594-66344

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Annexin A1 (CL594-66344, Clone:1E1B7) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: A549-Zellen
• Erfolgreiche Detektion in FC: K-562-Zellen, Jurkat-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66344 bindet in IF, FC (Intra) Annexin A1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Annexin A1 fusion protein Ag17273
• Vollständiger Name: annexin A1
• Berechnetes Molekulargewicht: 346 aa, 39 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC001275
• Gene symbol: ANXA1
• Gene ID (NCBI): 301
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes.

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C