Annexin A10 Monoklonaler Antikörper

Annexin A10 Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1A7A11

Kat-Nr. : 66869-1-Ig

Synonyme

Annexin 10, Annexin 14, annexin A10, ANX14, ANXA10

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  WB analysis of pig stomach using 66869-1-Ig

  pig stomach tissue were subjected to SDS PAGE followed by western blot with 66869-1-Ig (Annexin A10 antibody) at dilution of 1:2100 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-29 using 66869-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66869-1-Ig (Annexin A10 antibody) at dilution of 1:2100 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 66869-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 66869-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66869-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66869-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 66869-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 66869-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 66869-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 66869-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 66869-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 66869-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon cancer using 66869-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Annexin A10 antibody (66869-1-Ig, Clone: 1A7A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human colon cancer using 66869-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Annexin A10 antibody (66869-1-Ig, Clone: 1A7A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Haussschwein-Magengewebe, HT-29-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe, humanes Kolonkarzinomgewebe, humanes Kolongewebe, humanes Pankreaskarzinomgewebe, Maus-Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Kolonkarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66869-1-Ig bindet in WB, IHC, IF-P, ELISA Annexin A10 und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Annexin A10 fusion protein Ag27098
• Vollständiger Name: annexin A10
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC007320
• Gene symbol: ANXA10
• Gene ID (NCBI): 11199
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ANXA10, member of the Annexin family, is a calcium-and-phospholipid-binding protein. It is implicated in multiple physiological processes, including growth regulation, cell division, apoptosis, and differentiation. ANXA10 was recently observed to be upregulated in Barrett's esophagus, oral cancers, pancreatic cancer and colon cancer.

Publikationen

Species	Application	Title
human	WB	Discov Oncol; Identification of KRT16 and ANXA10 as cell cycle regulation genes for lung adenocarcinoma based on self-transcriptome sequencing of surgical samples and TCGA public data mining; Authors - Wen-Jian Liu