B23/NPM1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

B23/NPM1 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®555 Fluorescent Dye

Publikationen

1

CloneNo.

4F12A3

Kat-Nr. : CL555-60096

Synonyme

B23, B23/NPM1, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin, Numatrin

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Galerie der Validierungsdaten

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  IF Staining of HepG2 using CL555-60096

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®555 B23/NPM1 antibody (CL555-60096, Clone: 4F12A3 ) at dilution of 1:1000. DAPI (blue).

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  FC experiment of HeLa using CL555-60096

  1X10^6 HeLa cells were intracellularly stained with 0.25 ug CoraLite®555 Anti-Human B23/NPM1 (CL555-60096, Clone:4F12A3) (red), or 0.25 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:500-1:2000
• Durchflusszytometrie (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL555-60096 bindet in IF, FC (Intra) B23/NPM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: B23/NPM1 fusion protein Ag7415
• Vollständiger Name: nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 35-38 kDa
• GenBank-Zugangsnummer: BC002398
• Gene symbol: NPM1
• Gene ID (NCBI): 4869
• Konjugation: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.

Publikationen

Species	Application	Title
human	IF	iScience; Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges; Authors - Tamami Miyagi

Verwandte Produkte

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011