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Beta-2-Microglobulin Monoklonaler Antikörper

Beta-2-Microglobulin Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1C3B7

Kat-Nr. : 66207-1-Ig

Synonyme

B2M, Beta-2-microglobulin, HDCMA22P

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of various lysates using Beta-2-Microglobulin Monoclonal antibody (66207-1-Ig)

    WB analysis using 66207-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66207-1-Ig (Beta-2-Microglobulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using Beta-2-Microglobulin Monoclonal antibody (66207-1-Ig)

    WB analysis of Raji using 66207-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 66207-1-Ig (B2M Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Beta-2-Microglobulin Monoclonal antibody (66207-1-Ig)

    IHC staining of human prostate cancer using 66207-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66207-1-Ig (Beta-2-Microglobulin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Beta-2-Microglobulin Monoclonal antibody (66207-1-Ig)

    IHC staining of human prostate cancer using 66207-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66207-1-Ig (Beta-2-Microglobulin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using Beta-2-Microglobulin Monoclonal antibody (66207-1-Ig)

    IHC staining of human renal cell carcinoma using 66207-1-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66207-1-Ig (B2M Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using Beta-2-Microglobulin Monoclonal antibody (66207-1-Ig)

    IHC staining of human renal cell carcinoma using 66207-1-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66207-1-Ig (B2M Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using Beta-2-Microglobulin Monoclonal antibody (66207-1-Ig)

    IF Staining of human prostate cancer using 66207-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using Beta-2-Microglobulin antibody (66207-1-Ig, Clone: 1C3B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBA431-Zellen, LNCaP-Zellen, Raji-Zellen
Erfolgreiche Detektion in IHChumanes Prostatakarzinomgewebe, humanes Nierenzellkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFhumanes Prostatakarzinomgewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
Immunhistochemie (IHC)IHC : 1:250-1:1000
Immunfluoreszenz (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66207-1-Ig bindet in WB, IHC, IF, ELISA Beta-2-Microglobulin und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name beta-2-microglobulin
Berechnetes Molekulargewicht 14 kDa
Beobachtetes Molekulargewicht 13 kDa
GenBank-ZugangsnummerBC032589
Gene symbol B2M
Gene ID (NCBI) 567
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.

Protokolle

Produktspezifische Protokolle
WB protocol for Beta-2-Microglobulin antibody 66207-1-IgProtokoll herunterladen
IHC protocol for Beta-2-Microglobulin antibody 66207-1-IgProtokoll herunterladen
IF protocol for Beta-2-Microglobulin antibody 66207-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Santhosh (Verified Customer) (04-15-2019)

Human tonsil FFPE tissue rehydrated and antigen retrieval performed with citrate buffer.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:200
Beta-2-Microglobulin Antibody Immunohistochemistry, validation (1:200 dilution) in (Cat no:66207-1-Ig)