Beta-2-Microglobulin Monoklonaler Antikörper

Beta-2-Microglobulin Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

1C3B7

Rezensionen (1)

Kat-Nr. : 66207-1-Ig

Synonyme

B2M, 1C3B7, HDCMA22P, CDABP0092, Beta-2-microglobulin form pI 5.3

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  WB analysis of Raji using 66207-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66207-1-Ig (B2M Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66207-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66207-1-Ig (Beta-2-Microglobulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 66207-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66207-1-Ig (Beta-2-Microglobulin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66207-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66207-1-Ig (Beta-2-Microglobulin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 66207-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66207-1-Ig (B2M Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human renal cell carcinoma using 66207-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66207-1-Ig (B2M Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of human prostate cancer using 66207-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using Beta-2-Microglobulin antibody (66207-1-Ig, Clone: 1C3B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, LNCaP-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Nierenzellkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Prostatakarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

66207-1-Ig bindet in WB, IHC, IF-P, ELISA Beta-2-Microglobulin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Affe, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: beta-2-microglobulin
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 13 kDa
• GenBank-Zugangsnummer: BC032589
• Gene symbol: B2M
• Gene ID (NCBI): 567
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.

Publikationen

Species	Application	Title
mouse	WB	J Immunother Cancer; Targeting oxidative phosphorylation to increase the efficacy of immune-combination therapy in renal cell carcinoma; Authors - Jihua Tian
human	WB	J Agric Food Chem; Carnosic Acid: A Novel Selective Inhibitor of ERAP1 by Direct Binding and Its Modulation of Antigen Processing and Presentation; Authors - Jiaqi Wu
monkey	WB	J Virol; The neonatal Fc receptor (FcRn) is required for porcine reproductive and respiratory syndrome virus uncoating; Authors - Kang Yang
rat	WB	Int Immunopharmacol; Identification of potential targets regulating neutrophil extracellular traps in acute rejection of kidney transplantation based on transcriptomics and animal experiments; Authors - Jun Pei

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Santhosh (Verified Customer) (04-15-2019)

Human tonsil FFPE tissue rehydrated and antigen retrieval performed with citrate buffer.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200

Beta-2-Microglobulin Antibody Immunohistochemistry, validation (1:200 dilution) in (Cat no:66207-1-Ig)