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  • Featured Product
  • KD/KO Validated

BAP31 Polyklonaler Antikörper

BAP31 Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-11200

Synonyme

6C6 AG, BAP31, BCAP31, CDM, DXS1357E, p28, Protein CDM

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated BAP31 Polyclonal ant (CL488-11200)

    IF Staining of HeLa using CL488-11200

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 BAP31 antibody (CL488-11200) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated BAP31 Polyclonal ant (CL488-11200)

    FC experiment of HeLa using CL488-11200

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human BAP31 (CL488-11200) (red) or 0.4 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHeLa-Zellen
Erfolgreiche Detektion in FCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-11200 bindet in IF, FC (Intra) BAP31 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen BAP31 fusion protein Ag1668
Vollständiger Name B-cell receptor-associated protein 31
Berechnetes Molekulargewicht 28 kDa
Beobachtetes Molekulargewicht 28 kDa
GenBank-ZugangsnummerBC014323
Gene symbol BCAP31
Gene ID (NCBI) 10134
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BAP31 is a chaperone protein that is abundant in the endoplasmic reticulum (ER). BAP31 plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded protein and their targeting to the ER associated-degradation (ERAD). It also serves as a cargo receptor for the export of transmembrane proteins. BAP31 may be involved in apoptosis.

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 BAP31 antibody CL488-11200Protokoll herunterladen
FC protocol for CL Plus 488 BAP31 antibody CL488-11200Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen