BAX Monoklonaler Antikörper

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• KD/KO Validated

BAX Monoklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte und mehr (4)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(558)

CloneNo.

4G5E8

Kat-Nr. : 60267-1-Ig

Synonyme

4G5E8, Apoptosis regulator BAX, Bcl2-L-4, Bcl-2-like protein 4

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  WB analysis using 60267-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60267-1-Ig (BAX antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 60267-1-Ig

  EC109 cells were subjected to SDS PAGE followed by western blot with 60267-1-Ig (BAX antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60267-1-Ig

  WB result of BAX antibody (60267-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BAX transfected HeLa cells.

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  WB analysis of human testis using 60267-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 60267-1-Ig (BAX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 60267-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60267-1-Ig (BAX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of THP-1 using 60267-1-Ig

  IP result of anti-BAX (IP:60267-1-Ig, 4ug; Detection:60267-1-Ig 1:6000) with THP-1 cells lysate 1520 ug.

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  IHC staining of human liver cancer using 60267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 60267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 60267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 60267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 60267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 60267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human rectal cancer using 60267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 60267-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using BAX antibody (60267-1-Ig, Clone: 4G5E8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, COLO 320-Zellen, EC109-Zellen, HEK-293-Zellen, HepG2-Zellen, humanes Hodengewebe, Neuro-2a-Zellen, PC-12-Zellen, ROS1728-Zellen
• Erfolgreiche IP: THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Nierengewebe, humanes Lungenkarzinomgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 531 publications below
• IHC: See 29 publications below
• CoIP: See 1 publications below

Produktinformation

60267-1-Ig bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA BAX und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Hausschwein, Hund, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BAX fusion protein Ag21068
• Vollständiger Name: BCL2-associated X protein
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC014175
• Gene symbol: BAX
• Gene ID (NCBI): 581
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BAX, also named as BCL2L4, is a pro-apoptotic member of the Bcl-2 protein family, which plays a pivotal role in controlling cell life and death. Bax largely localizes to the cytoplasm of healthy cells, but accumulates on the outer mitochondrial membrane upon apoptosis induction (PMID: 9108035). BAX can commit a cell to apoptosis by translocation from the cytosol to the mitochondria and permeabilization of the outer mitochondrial membrane, which leads to the release of cytochrome c from mitochondria (PMID: 21763611). The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis (PMID: 8183579).

Publikationen

Species	Application	Title
human	WB	Adv Sci (Weinh); Mitochondrial tRNAGlu 14693A>G Mutation, an "Enhancer" to the Phenotypic Expression of Leber's Hereditary Optic Neuropathy; Authors - Lihao Jin
rat,mouse	WB,IHC	Acta Pharm Sin B; Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.; Authors - Xunxun Wu
human	WB	Cell Death Differ; ZNF451 collaborates with RNF8 to regulate RNF168 localization and amplify ubiquitination signaling to promote DNA damage repair and regulate radiosensitivity; Authors - Feng Xu
rat	WB	J Adv Res; Evolocumab prevents atrial fibrillation in rheumatoid arthritis rats through restraint of PCSK9 induced atrial remodeling; Authors - Xuejie Han
	WB	J Nanobiotechnology; Nanocomposites based on nanoceria regulate the immune microenvironment for the treatment of polycystic ovary syndrome; Authors - Sisi Yan
human	WB	Redox Biol; Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.; Authors - Zhongren Xu