BAX Monoklonaler Antikörper

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• KD/KO Validated

BAX Monoklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, IF, ICC, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

352

CloneNo.

4G5E8

Kat-Nr. : 60267-1-Ig

Synonyme

Apoptosis regulator BAX, BAX

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Galerie der Validierungsdaten

• 

  WB analysis using 60267-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60267-1-Ig (BAX antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 60267-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 60267-1-Ig (BAX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 60267-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60267-1-Ig (BAX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of THP-1 using 60267-1-Ig

  IP result of anti-BAX(IP:60267-1-Ig, 4ug; Detection:60267-1-Ig 1:6000) with THP-1 cells lysate 1520 ug.

• 

  IHC staining of human liver cancer using 60267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 60267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of MCF-7 using 60267-1-Ig

  1X10^6 MCF-7 cells were stained with 0.2 ug Anti-Human BAX (60267-1-Ig, Clone:4G5E8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, COLO 320-Zellen, HEK-293-Zellen, HepG2-Zellen, humanes Hodengewebe, Neuro-2a-Zellen, PC-12-Zellen, ROS1728-Zellen
• Erfolgreiche IP: THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 335 publications below
• IHC: See 20 publications below
• IF: See 17 publications below
• CoIP: See 1 publications below

Produktinformation

60267-1-Ig bindet in WB, IP, IHC, IF, ICC, FC, CoIP, ELISA BAX und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Hund, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BAX fusion protein Ag21068
• Vollständiger Name: BCL2-associated X protein
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC014175
• Gene symbol: BAX
• Gene ID (NCBI): 581
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BAX, also named as BCL2L4, is a pro-apoptotic member of the Bcl-2 protein family, which plays a pivotal role in controlling cell life and death. Bax largely localizes to the cytoplasm of healthy cells, but accumulates on the outer mitochondrial membrane upon apoptosis induction (PMID: 9108035). BAX can commit a cell to apoptosis by translocation from the cytosol to the mitochondria and permeabilization of the outer mitochondrial membrane, which leads to the release of cytochrome c from mitochondria (PMID: 21763611). The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis (PMID: 8183579).

Publikationen

Species	Application	Title
rat	WB,ICC	Acta Pharm Sin B; Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.; Authors - Xunxun Wu
rat	WB	J Adv Res; Evolocumab prevents atrial fibrillation in rheumatoid arthritis rats through restraint of PCSK9 induced atrial remodeling; Authors - Xuejie Han
human	WB	Redox Biol; Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.; Authors - Zhongren Xu
mouse	WB,IF	Cell Death Dis; DUSP1 protects against ischemic acute kidney injury through stabilizing mtDNA via interaction with JNK; Authors - Lang Shi
mouse	WB	Theranostics; Proteomic analysis reveals ginsenoside Rb1 attenuates myocardial ischemia/reperfusion injury through inhibiting ROS production from mitochondrial complex I.; Authors - Lujing Jiang
mouse	WB	Cell Prolif; OGG1 aggravates renal ischemia-reperfusion injury by repressing PINK1-mediated mitophagy; Authors - Fan Zhao

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