BCHE Polyklonaler Antikörper

BCHE Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 23854-1-AP

Synonyme

Acylcholine acylhydrolase, BCHE, Butyrylcholine esterase, butyrylcholinesterase, CHE1, Choline esterase II, Cholinesterase, E1, Pseudocholinesterase

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  WB analysis of HeLa using 23854-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23854-1-AP (BCHE Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 23854-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23854-1-AP (BCHE Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 23854-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23854-1-AP (BCHE Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 23854-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23854-1-AP (BCHE Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 23854-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using BCHE antibody (23854-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

23854-1-AP bindet in WB, IHC, IF/ICC, ELISA BCHE und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BCHE fusion protein Ag20888
• Vollständiger Name: butyrylcholinesterase
• Berechnetes Molekulargewicht: 602 aa, 68 kDa
• Beobachtetes Molekulargewicht: 85-90 kDa
• GenBank-Zugangsnummer: BC008396
• Gene symbol: BCHE
• Gene ID (NCBI): 590
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BCHE(butyrylcholinesterase) is an enzyme expressed in most human tissues. It may have a greater role in cholinergic transmission than previously surmised, making BChE inhibition an important therapeutic goal in Alzheimer's disease(PMID:11848688). BCHE is also named as CHE1 and belongs to the type-B carboxylesterase/lipase family. This is a glycoprotein with the molecular weight from 68 kDa to 90 kDa.

Publikationen

Species	Application	Title
human	WB	Nat Biomed Eng; Genome-edited skin epidermal stem cells protect mice from cocaine-seeking behaviour and cocaine overdose.; Authors - Yuanyuan Li
goat	WB,IF	Cells; Improving the Efficiency of Precise Genome Editing with CRISPR/Cas9 to Generate Goats Overexpressing Human Butyrylcholinesterase; Authors - Jia-Hao Wang
human	WB,IHC	Front Immunol; Progressions of the correlation between lipid metabolism and immune infiltration characteristics in gastric cancer and identification of BCHE as a potential biomarker; Authors - Shibo Wang