BCL2 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

BCL2 Monoklonal Antikörper für IF/ICC, IF-P

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human

Anwendung

IF/ICC, IF-P

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4H8C6

Kat-Nr. : CL488-60178

Synonyme

BCL-2, 4H8C6

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  IF Staining of human tonsillitis using CL488-60178

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488-conjugated BCL2 antibody (CL488-60178, Clone: 4H8C6 ) at dilution of 1:100.

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  IF Staining of human tonsillitis using CL488-60178

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488-conjugated BCL2 antibody (CL488-60178, Clone: 4H8C6 ) at dilution of 1:100.

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  IF Staining of human appendicitis using CL488-60178

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite® Plus 488 BCL2 antibody (CL488-60178, Clone: 4H8C6 ) at dilution of 1:200.

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  IF Staining of human appendicitis using CL488-60178

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite® Plus 488 BCL2 antibody (CL488-60178, Clone: 4H8C6 ) at dilution of 1:200.

• 

  IF Staining of MCF-7 using CL488-60178

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 BCL2 antibody (CL488-60178, Clone: 4H8C6 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Tonsillitisgewebe, humanes Appendizitis-Gewebe
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-60178 bindet in IF/ICC, IF-P BCL2 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BCL2 fusion protein Ag3508
• Vollständiger Name: B-cell CLL/lymphoma 2
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC027258
• Gene symbol: BCL2
• Gene ID (NCBI): 596
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BCL2 belongs to the Bcl-2 family. It suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. BCL2 regulates cell death by controlling the mitochondrial membrane permeability. It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).