BCL2L13 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

BCL2L13 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Rezensionen (1)

Kat-Nr. : 16612-1-AP

Synonyme

BCL2L13, MIL1, Protein Mil1

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Galerie der Validierungsdaten

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  WB analysis using 16612-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16612-1-AP (BCL2L13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16612-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16612-1-AP (BCL2L13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 16612-1-AP

  IP Result of anti-BCL2L13 (IP:16612-1-AP, 3ug; Detection:16612-1-AP 1:100) with mouse heart tissue lysate 8000ug.

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  IHC staining of human pancreas cancer using 16612-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16612-1-AP (BCL2L13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 16612-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16612-1-AP (BCL2L13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Produktinformation

16612-1-AP bindet in WB, IP, IHC, IF, ELISA BCL2L13 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BCL2L13 fusion protein Ag9841
• Vollständiger Name: BCL2-like 13 (apoptosis facilitator)
• Berechnetes Molekulargewicht: 485 aa, 53 kDa
• Beobachtetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: BC003032
• Gene symbol: BCL2L13
• Gene ID (NCBI): 23786
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BCL2L13/MIL1 gene encodes a mitochondrially-localized protein with conserved B-cell lymphoma 2 homology motifs. BLC2L13 gene is highest expressed in heart, placenta and pancreas. The encoded protein Bcl-rambo may promote the activation of caspase-3 and apoptosis. The Bcl-rambo cell death activity was induced by its membrane-anchored C-terminal domain and not by the Bcl-2 homology region (PMID: 11262395). Multivariate analysis demonstrated that high expression of BCL2L13 was an independent prognostic factor for in childhood acute lymphoblastic leukemia (PMID: 20109966).

Publikationen

Species	Application	Title
mouse	IHC	Cancer Commun (Lond); Glucose-mediated mitochondrial reprogramming by cholesterol export at TM4SF5-enriched mitochondria-lysosome contact sites; Authors - Ji Eon Kim
human	WB	Nat Commun; Bcl-2-like protein 13 is a mammalian Atg32 homologue that mediates mitophagy and mitochondrial fragmentation.; Authors - Tomokazu Murakawa
human,mouse	WB	Autophagy; BNIP3L/NIX degradation leads to mitophagy deficiency in ischemic brains.; Authors - Xiaoli Wu
mouse	WB	Autophagy; BNIP3L/Nix-induced mitochondrial fission, mitophagy, and impaired myocyte glucose uptake are abrogated by PRKA/PKA phosphorylation.; Authors - Simone C da Silva Rosa
human	WB,IHC,IF	Proc Natl Acad Sci U S A; Bcl2L13 is a ceramide synthase inhibitor in glioblastoma.; Authors - Samuel A Jensen ; KD Validated
mouse,rat	IF	Glia; Neuroglial transmitophagy and Parkinson's disease.; Authors - Ingrid Morales

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Melissa (Verified Customer) (07-28-2022)

Antibody worked well in western blot. Lysate used was from Huh7 cells IP-ed for a different target protein.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Huh7 cells

BCL2L13 Antibody Western Blot validation (1:1000 dilution) in Huh7 cells (Cat no:16612-1-AP)