BCL6 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

BCL6 Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 21187-1-AP

Synonyme

B cell CLL/lymphoma 6, B cell lymphoma 5 protein, B cell lymphoma 6 protein, BCL6, LAZ3, Protein LAZ 3, ZBTB27, Zinc finger protein 51, ZNF51

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Galerie der Validierungsdaten

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  WB analysis of Raji using 21187-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21187-1-AP (BCL6 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 21187-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21187-1-AP (BCL6 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 21187-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21187-1-AP (BCL6 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 21187-1-AP

  IP Result of anti-BCL6 (IP:21187-1-AP, 4ug; Detection:21187-1-AP 1:1000) with Raji cells lysate 1400ug.

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  IHC staining of human lymphoma using 21187-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 21187-1-AP (BCL6 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human tonsillitis using 21187-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21187-1-AP (BCL6 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human tonsillitis using 21187-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21187-1-AP (BCL6 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  FC experiment of Ramos using 21187-1-AP

  1X10^6 Ramos cells were intracellularly stained with 0.2 ug Anti-Human BCL6 (21187-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 4 publications below
• IP: See 1 publications below

Produktinformation

21187-1-AP bindet in WB, IP, IHC, FC, ELISA BCL6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BCL6 fusion protein Ag15519
• Vollständiger Name: B-cell CLL/lymphoma 6
• Berechnetes Molekulargewicht: 706 aa, 79 kDa
• Beobachtetes Molekulargewicht: 79-95 kDa
• GenBank-Zugangsnummer: BC150184
• Gene symbol: BCL6
• Gene ID (NCBI): 604
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BCl6, a zinc finger transcription factor, contains a N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages.

Publikationen

Species	Application	Title
	IHC	Nat Commun; A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice.; Authors - Christine Quach
human	WB	Respir Res; Transcriptional inhibition of miR-486-3p by BCL6 upregulates Snail and induces epithelial-mesenchymal transition during radiation-induced pulmonary fibrosis.; Authors - Ziyan Yan
mouse	WB	Sci Rep; Specific immunotherapy in combination with Clostridium butyricum inhibits allergic inflammation in the mouse intestine.; Authors - Yanhong Shi
human	WB,IP	Front Cell Dev Biol; Development of a contacting transwell co-culture system for the in vitro propagation of primary central nervous system lymphoma; Authors - Mayuko Nishi
human	IHC	Front Cell Dev Biol; The Construction and Analysis of ceRNA Network and Patterns of Immune Infiltration in Colon Adenocarcinoma Metastasis.; Authors - Zhengyan Chang
human	IHC	Front Oncol; Whole-Exome Sequencing Reveals New Potential Mutations Genes for Primary Mucosa-Associated Lymphoid Tissue Lymphoma Arising From the Kidney.; Authors - Shuang Wen

Verwandte Produkte

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011