BCR Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

BCR Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Rezensionen (1)

Kat-Nr. : 22585-1-AP

Synonyme

ALL, BCR, breakpoint cluster region, CML, D22S11, D22S662, PHL

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Galerie der Validierungsdaten

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  WB analysis of Y79 using 22585-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22585-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 22585-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22585-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 22585-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COS-7 using 22585-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 22585-1-AP

  IP Result of anti-BCR (IP:22585-1-AP, 4ug; Detection:22585-1-AP 1:1000) with HEK-293 cells lysate 3000ug.

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  IHC staining of human skin using 22585-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 22585-1-AP (BCR Antibody) at dilution of 1:50.

• 

  IHC staining of human skin using 22585-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 22585-1-AP (BCR Antibody) at dilution of 1:50.

• 

  IF Staining of HeLa using 22585-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22585-1-AP (BCR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 22585-1-AP

  Immunofluorescent analysis of HepG2 cells, using BCR antibody 22585-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HepG2 using 22585-1-AP

  1X10^6 HepG2 cells were stained with .2ug BCR antibody (22585-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen, COS-7-Zellen, HEK-293-Zellen, HeLa-Zellen, K-562-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

22585-1-AP bindet in WB, IP, IHC, IF, FC, ELISA BCR und zeigt Reaktivität mit Affe, human

• Getestete Reaktivität: Affe, human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BCR fusion protein Ag18281
• Vollständiger Name: breakpoint cluster region
• Berechnetes Molekulargewicht: 143 kDa
• Beobachtetes Molekulargewicht: 150-160 kDa
• GenBank-Zugangsnummer: BC066122
• Gene symbol: BCR
• Gene ID (NCBI): 613
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Bcr gene, mapping on chromosome22, was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The Bcr gene encodes for the break point cluster region protein(Bcr). Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for RAC1 and CDC42. A chromosomal aberration involving BCR has been found in patients with chronic myeloid leukemia.

Publikationen

Species	Application	Title
human,mouse	WB,IHC	Neurogastroenterol Motil; Loss function of Bcr mutation causes gastrointestinal dysmotility and brain developmental defects.; Authors - Yongtao Xiao ; KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Costanza (Verified Customer) (09-27-2022)

Good Immunofluorescence from paraffin sections

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/100