BCR Monoklonaler Antikörper

BCR Monoklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Ratte

Anwendung

WB, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2C2E3

Kat-Nr. : 67260-1-Ig

Synonyme

ALL, BCR, breakpoint cluster region, CML, D22S11, D22S662, FLJ16453, PHL

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 67260-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67260-1-Ig (BCR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67260-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67260-1-Ig (BCR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67260-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67260-1-Ig (BCR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of K-562 using 67260-1-Ig

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human BCR (67260-1-Ig, Clone:2C2E3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in FC: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67260-1-Ig bindet in WB, FC, ELISA BCR und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BCR fusion protein Ag28953
• Vollständiger Name: breakpoint cluster region
• Berechnetes Molekulargewicht: 143 kDa
• Beobachtetes Molekulargewicht: 150-160 kDa
• GenBank-Zugangsnummer: BC066122
• Gene symbol: BCR
• Gene ID (NCBI): 613
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Bcr gene, mapping on chromosome22, was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The Bcr gene encodes for the break point cluster region protein(Bcr). Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for RAC1 and CDC42. A chromosomal aberration involving BCR has been found in patients with chronic myeloid leukemia.