BCS1L Monoklonaler Antikörper

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• KD/KO Validated

BCS1L Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

3G6H1

Kat-Nr. : 60212-1-Ig

Synonyme

BCS1L, BJS, FLNMS, GRACILE, h BCS, h BCS1, Hs.6719, Mitochondrial chaperone BCS1, PTD

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 60212-1-Ig

  WB result of BCS1L antibody (60212-1-Ig; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCS1L transfected HEK-293 cells.

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  WB analysis of COLO 320 using 60212-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60212-1-Ig (BCS1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 60212-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas using 60212-1-Ig(BCS1L antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 60212-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas using 60212-1-Ig(BCS1L antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 60212-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 60212-1-Ig(BCS1L antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 60212-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 60212-1-Ig(BCS1L antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 60212-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 60212-1-Ig(BCS1L antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 60212-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 60212-1-Ig(BCS1L antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MCF-7 using 60212-1-Ig

  Immunofluorescent analysis of () fixed MCF-7 cells using 60212-1-Ig (BCS1L antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Colo320 cells, COLO 320-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Hirngewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

60212-1-Ig bindet in WB, IHC, IF, ELISA BCS1L und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BCS1L fusion protein Ag18280
• Vollständiger Name: BCS1-like (yeast)
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC007500
• Gene symbol: BCS1L
• Gene ID (NCBI): 617
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human BCS1-like (BCS1L), a mitochondrial inner-membrane protein, is a chaperone necessary for the assembly of mitochondrial respiratory chain complex III. Recently studies indicated that the mutations in this protein may cause the GRACILE (growth retardation, aminoaciduria, cholestasis, iron overload, lactacidosis, and early death) syndrome, a recessively inherited lethal disease characterized by fetal growth retardation, lactic acidosis, aminoaciduria, cholestasis, and abnormalities in iron metabolism.