BDH1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

BDH1 Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

2C3D4

Kat-Nr. : 67448-1-Ig

Synonyme

2C3D4, 3-hydroxybutyrate dehydrogenase, BDH, D-beta-hydroxybutyrate dehydrogenase, mitochondrial, EC:1.1.1.30

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  WB analysis of pig liver using 67448-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67448-1-Ig (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67448-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67448-1-Ig (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67448-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67448-1-Ig (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-29 using 67448-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67448-1-Ig (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 67448-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 67448-1-Ig (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67448-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67448-1-Ig (BDH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67448-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67448-1-Ig (BDH1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse liver using 67448-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using BDH1 antibody (67448-1-Ig, Clone: 2C3D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse liver using 67448-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using BDH1 antibody (67448-1-Ig, Clone: 2C3D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Lebergewebe, COLO 320-Zellen, HepG2-Zellen, HT-29-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mauslebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

67448-1-Ig bindet in WB, IHC, IF-P, ELISA BDH1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BDH1 fusion protein Ag7864
• Vollständiger Name: 3-hydroxybutyrate dehydrogenase, type 1
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC005844
• Gene symbol: BDH1
• Gene ID (NCBI): 622
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BDH1 (3-hydroxybutyrate dehydrogenase, type 1) is a 343 amino acid protein that localizes to the mitochondrial matrix and belongs to the short-chain dehydrogenases/reductases (SDR) family. The deduced 343 amino acid protein contains a 46 residue leader peptide, which is cleaved to produce the mature form. It is a lipid-requiring mitochondrial enzyme with a specific requirement of phosphatidylcholine (PC).

Publikationen

Species	Application	Title
mouse,human	WB,IHC	MedComm (2020); BDH1-mediated LRRC31 regulation dependent on histone lysine β-hydroxybutyrylation to promote lung adenocarcinoma progression; Authors - Jingjing Huang ; KD Validated
mouse,human	WB	bioRxiv; An alternative route for β-hydroxybutyrate metabolism supports fatty acid synthesis in cancer cells; Authors - Faith C Kaluba ; KD Validated
mouse	WB	Acta Pharm Sin B; Parabacteroides distasonis promotes liver regeneration by increasing β-hydroxybutyric acid (BHB) production and BHB-driven STAT3 signals; Authors - Manlan Guo
mouse	WB	PNAS Nexus; Frataxin controls ketone body metabolism through regulation of OXCT1; Authors - Yi NA Dong