• Featured Product
  • KD/KO Validated

Beclin 1 Polyklonaler Antikörper

Beclin 1 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IF-Fro, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (6)

Anwendung

WB, IHC, IF/ICC, IF-P, IF-Fro, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(1107)

Kat-Nr. : 11306-1-AP

Synonyme

BECN1, Beclin-1-C 37 kDa, Beclin-1-C 35 kDa, Beclin-1, Beclin1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBC6-Zellen, A431-Zellen, HEK-293-Zellen, HeLa-Zellen, HT-1080.Zellen, humanes Plazenta-Gewebe, L02-Zellen, MDA-MB-453s-Zellen, Maushirngewebe, NIH/3T3-Zellen, PC-3-Zellen, Raji-Zellen, Rattenhirngewebe, SH-SY5Y-Zellen
Erfolgreiche IPMDA-MB-453s-Zellen
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe, humanes Mammahyperplasie-Gewebe, humanes Zervixkarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Prostatahyperplasie-Gewebe, humanes Magengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-PMausherzgewebe
Erfolgreiche Detektion in IF-FroMausherzgewebe
Erfolgreiche Detektion in IF/ICCNIH/3T3-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:10000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)-PIF-P : 1:50-1:500
Immunfluoreszenz (IF)-FROIF-FRO : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

11306-1-AP bindet in WB, IHC, IF/ICC, IF-P, IF-Fro, IP, CoIP, ELISA Beclin 1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Fisch, hamster, Hausschwein, Huhn, Maus, Ratte, Zebrafisch, Ziege
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Beclin 1 fusion protein Ag1843
Vollständiger Name beclin 1, autophagy related
Berechnetes Molekulargewicht 52 kDa
Beobachtetes Molekulargewicht52-60 kDa
GenBank-ZugangsnummerBC010276
Gene symbol Beclin 1
Gene ID (NCBI) 8678
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Beclin 1, also known as ATG6 or VPS30, interacts with various cofactors (e.g. Ambra1, Barkor (Atg14), Rubicon, or UVRAG) to regulate the lipid kinase Vps34 and promote the formation of the BECLIN1-Vps34-Vps15 complex, hence inducing autophagy. Its function (via the BH3 domain) is inhibited by Bcl-2 or Bcl-XL. Beclin 1 (BECN1) is a crucial molecule in the control of the autophagic activity, and its activity is regulated by multiple mechanisms, including the post-translational modification, protein-protein interaction, and subcellular localization. It plays a role in crosstalk between apoptosis and autophagy. It has been reported that Beclin 1 can be cleaved into fragments of 50, 37 and 35 kDa during apoptosis. It is involved in many disorders, including neurodegeneration and cancer (tumorigenesis). Beclin 1 is a mammalian tumor suppressor, and its gene is monoallelically deleted in 75% of ovarian, 50% of breast, and 40% of prostate cancers. Decreased expression of Beclin 1 has also been observed in human brain and lung tumors. The level of Beclin 1 was decreased in the affected brain regions of patients with Alzheimer's disease early in the disease process. Recent studies have also shown that gain and loss of Beclin 1 function affects the death of heart cells.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Beclin 1 antibody 11306-1-APProtokoll herunterladen
IHC protocol for Beclin 1 antibody 11306-1-APProtokoll herunterladenl
IF protocol for Beclin 1 antibody 11306-1-APProtokoll herunterladen
IP protocol for Beclin 1 antibody 11306-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Cell

TEX264 drives selective autophagy of DNA lesions to promote DNA repair and cell survival

Authors - Pauline Lascaux
humanWB,IP

Signal Transduct Target Ther

Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy

Authors - Yang Meng
humanWB

Mol Cancer

Overcoming multi-drug resistance in SCLC: a synergistic approach with venetoclax and hydroxychloroquine targeting the lncRNA LYPLAL1-DT/BCL2/BECN1 pathway

Authors - Shuxin Li
humanWB

Nat Microbiol

Kaposi's sarcoma-associated herpesvirus induces mitochondrial fission to evade host immune responses and promote viral production

Authors - Qing Zhu
mouseIF

ACS Nano

Neutrophil Nanovesicle Protects against Experimental Autoimmune Encephalomyelitis through Enhancing Myelin Clearance by Microglia

Authors - Shishi Shen
humanWB,IHC

Cancer Commun (Lond)

Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies

Authors - Lu Tang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Jenna (Verified Customer) (07-28-2025)

The product was easy to use and provided all necessary information for successful completion of western blot procedures. The recommended amount did turn out to be the appropriate amount for our experimental purpose. Our success with this product encourages us to continue to use this product

  • Applications: Western Blot
  • Cell Tissue Type: Liver
FH

James (Verified Customer) (11-30-2022)

This antibody works well in WB

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Liver
Beclin 1 Antibody Western Blot validation (1/1000 dilution) in Liver (Cat no:11306-1-AP)
FH

Jonathan (Verified Customer) (04-08-2022)

Antibody worked well - gave a nice, specific and strong signal. Happy.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 in 1000
  • Cell Tissue Type: Vero cells (African Green Monkey)
FH

Zhengnan (Verified Customer) (02-24-2022)

The antibody is very good, got correct bands and background is clear!

  • Applications: Western Blot
FH

David (Verified Customer) (03-30-2020)

Used for immunoblot, single band at expected molecular weight, good signal to noise ratio

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: SH-SY5Y
FH

Kanika (Verified Customer) (09-18-2019)

Works well at the recommended dilution for western blotting.Immunofluorescence signal was variable/low even with same concentration in replicate experiments.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human platelets
Beclin 1 Antibody Western Blot,Immunofluorescence, validation (1:1000 dilution) in Human platelets (Cat no:11306-1-AP)
FH

Kishor (Verified Customer) (01-30-2019)

Working well for Western Blotting (1:5000) and IF (1:300)

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: H69, HUCCT-1
Beclin 1 Antibody Western Blot,Immunofluorescence, validation (1:1000 dilution) in H69, HUCCT-1 (Cat no:11306-1-AP)