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Beclin 1 Polyklonaler Antikörper
Beclin 1 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IF-Fro, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (6)
Anwendung
WB, IHC, IF/ICC, IF-P, IF-Fro, IP, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 11306-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | C6-Zellen, A431-Zellen, HEK-293-Zellen, HeLa-Zellen, HT-1080.Zellen, humanes Plazenta-Gewebe, L02-Zellen, MDA-MB-453s-Zellen, Maushirngewebe, NIH/3T3-Zellen, PC-3-Zellen, Raji-Zellen, Rattenhirngewebe, SH-SY5Y-Zellen |
Erfolgreiche IP | MDA-MB-453s-Zellen |
Erfolgreiche Detektion in IHC | humanes Mammakarzinomgewebe, humanes Mammahyperplasie-Gewebe, humanes Zervixkarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Prostatahyperplasie-Gewebe, humanes Magengewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF-P | Mausherzgewebe |
Erfolgreiche Detektion in IF-Fro | Mausherzgewebe |
Erfolgreiche Detektion in IF/ICC | NIH/3T3-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:1000-1:10000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:50-1:500 |
Immunfluoreszenz (IF)-P | IF-P : 1:50-1:500 |
Immunfluoreszenz (IF)-FRO | IF-FRO : 1:50-1:500 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 24 publications below |
WB | See 1011 publications below |
IHC | See 113 publications below |
IF | See 93 publications below |
IP | See 10 publications below |
ELISA | See 1 publications below |
CoIP | See 9 publications below |
Produktinformation
11306-1-AP bindet in WB, IHC, IF/ICC, IF-P, IF-Fro, IP, CoIP, ELISA Beclin 1 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Fisch, hamster, Hausschwein, Huhn, Maus, Ratte, Zebrafisch, Ziege |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | Beclin 1 fusion protein Ag1843 |
Vollständiger Name | beclin 1, autophagy related |
Berechnetes Molekulargewicht | 52 kDa |
Beobachtetes Molekulargewicht | 52-60 kDa |
GenBank-Zugangsnummer | BC010276 |
Gene symbol | Beclin 1 |
Gene ID (NCBI) | 8678 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Beclin 1, also known as ATG6 or VPS30, interacts with various cofactors (e.g. Ambra1, Barkor (Atg14), Rubicon, or UVRAG) to regulate the lipid kinase Vps34 and promote the formation of the BECLIN1-Vps34-Vps15 complex, hence inducing autophagy. Its function (via the BH3 domain) is inhibited by Bcl-2 or Bcl-XL. Beclin 1 (BECN1) is a crucial molecule in the control of the autophagic activity, and its activity is regulated by multiple mechanisms, including the post-translational modification, protein-protein interaction, and subcellular localization. It plays a role in crosstalk between apoptosis and autophagy. It has been reported that Beclin 1 can be cleaved into fragments of 50, 37 and 35 kDa during apoptosis. It is involved in many disorders, including neurodegeneration and cancer (tumorigenesis). Beclin 1 is a mammalian tumor suppressor, and its gene is monoallelically deleted in 75% of ovarian, 50% of breast, and 40% of prostate cancers. Decreased expression of Beclin 1 has also been observed in human brain and lung tumors. The level of Beclin 1 was decreased in the affected brain regions of patients with Alzheimer's disease early in the disease process. Recent studies have also shown that gain and loss of Beclin 1 function affects the death of heart cells.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for Beclin 1 antibody 11306-1-AP | Protokoll herunterladen |
IHC protocol for Beclin 1 antibody 11306-1-AP | Protokoll herunterladenl |
IF protocol for Beclin 1 antibody 11306-1-AP | Protokoll herunterladen |
IP protocol for Beclin 1 antibody 11306-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Cell TEX264 drives selective autophagy of DNA lesions to promote DNA repair and cell survival | ||
Signal Transduct Target Ther Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy | ||
Mol Cancer Overcoming multi-drug resistance in SCLC: a synergistic approach with venetoclax and hydroxychloroquine targeting the lncRNA LYPLAL1-DT/BCL2/BECN1 pathway | ||
Nat Microbiol Kaposi's sarcoma-associated herpesvirus induces mitochondrial fission to evade host immune responses and promote viral production | ||
ACS Nano Neutrophil Nanovesicle Protects against Experimental Autoimmune Encephalomyelitis through Enhancing Myelin Clearance by Microglia | ||
Cancer Commun (Lond) Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Jenna (Verified Customer) (07-28-2025) | The product was easy to use and provided all necessary information for successful completion of western blot procedures. The recommended amount did turn out to be the appropriate amount for our experimental purpose. Our success with this product encourages us to continue to use this product
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FH James (Verified Customer) (11-30-2022) | This antibody works well in WB
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FH Jonathan (Verified Customer) (04-08-2022) | Antibody worked well - gave a nice, specific and strong signal. Happy.
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FH Zhengnan (Verified Customer) (02-24-2022) | The antibody is very good, got correct bands and background is clear!
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FH David (Verified Customer) (03-30-2020) | Used for immunoblot, single band at expected molecular weight, good signal to noise ratio
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FH Kanika (Verified Customer) (09-18-2019) | Works well at the recommended dilution for western blotting.Immunofluorescence signal was variable/low even with same concentration in replicate experiments.
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FH Kishor (Verified Customer) (01-30-2019) | Working well for Western Blotting (1:5000) and IF (1:300)
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