Bestrophin-1 Monoklonaler Antikörper

Bestrophin-1 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

4D5F1

Kat-Nr. : 60326-1-Ig

Synonyme

ARB, BEST, BEST1, bestrophin 1, Bestrophin-1, BMD, TU15B, VMD2

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  WB analysis of Y79 using 60326-1-Ig

  Y79 cells were subjected to SDS PAGE followed by western blot with 60326-1-Ig (Bestrophin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 60326-1-Ig

  WB result of Bestrophin-1 antibody (60326-1-Ig; 1:1000; room temperature for 1.5 hours) with negatove control and Bestrophin-1 overexpressed Transfected HEK-293 cells.

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  IF Staining of Y79 using 60326-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Y79 cells using Bestrophin-1 antibody (60326-1-Ig, Clone: 4D5F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen, Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in IF/ICC: Y79-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

60326-1-Ig bindet in WB, IF/ICC, ELISA Bestrophin-1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Bestrophin-1 fusion protein Ag15129
• Vollständiger Name: bestrophin 1
• Berechnetes Molekulargewicht: 585 aa, 68 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC015220
• Gene symbol: Bestrophin/BEST1
• Gene ID (NCBI): 7439
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Bestrophin-1 (BEST1) is a 68 kDa transmembrane protein which belongs to the bestrophin family of anion channels. It is predominantly expressed in the basolateral membrane of the retinal pigment epithelium. Studies show that bestrophin 1 functions as a Ca(2+)-dependent Cl(-) channel and modulator of voltage-dependent L-type Ca(2+) channels. It may also contribute to the basolateral cell conductance in airway epithelial cells. This protein is encoded by the VMD2 gene. Mutations in VMD2 can lead to different types of retinal or macular degenerations, including Best vitelliform macular dystrophy (BMD), autosomal recessive bestrophinopathy (ARB), autosomal dominant vitreoretinochoroidopathy (ADVIRC) and adult-onset vitelliform macular dystrophy (AVMD).

Publikationen

Species	Application	Title
human	IF	Cell Death Dis; Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal degeneration; Authors - Xiaoman Zhu
human,rat	WB	Neurobiol Dis; Best1 mitigates ER stress induced by the increased cellular microenvironment stiffness in epilepsy; Authors - Hao Wu