BIN3 Polyklonaler Antikörper

BIN3 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20186-1-AP

Synonyme

BIN3, bridging integrator 3

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Galerie der Validierungsdaten

• 

  WB analysis of Jurkat using 20186-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20186-1-AP (BIN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20186-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20186-1-AP (BIN3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 20186-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20186-1-AP (BIN3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20186-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20186-1-AP (BIN3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 20186-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 20186-1-AP (BIN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, A375-Zellen, HEK-293-Zellen, HeLa-Zellen, Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

20186-1-AP bindet in WB, ELISA BIN3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BIN3 fusion protein Ag14091
• Vollständiger Name: bridging integrator 3
• Berechnetes Molekulargewicht: 253 aa, 30 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC001223
• Gene symbol: BIN3
• Gene ID (NCBI): 55909
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BIN3, also named as MEPCE and BCDIN3, belongs to the methyltransferase superfamily. It is an S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to stabilize it.