BLMH Polyklonaler Antikörper

BLMH Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 14941-1-AP

Synonyme

BH, bleomycin hydrolase, BLM hydrolase, BLMH, BMH

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Galerie der Validierungsdaten

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  WB analysis of human colon using 14941-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 14941-1-AP (BLMH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 14941-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14941-1-AP (BLMH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 14941-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14941-1-AP (BLMH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skin using 14941-1-AP

  IP Result of anti-BLMH (IP:14941-1-AP, 4ug; Detection:14941-1-AP 1:1000) with mouse skin tissue lysate 3200ug.

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  IHC staining of human colon cancer using 14941-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14941-1-AP (BLMH antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 14941-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14941-1-AP (BLMH antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 14941-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14941-1-AP (BLMH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 14941-1-AP

  Immunofluorescent analysis of Hela cells, using BLMH antibody 14941-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Kolongewebe, Mausmilzgewebe, Maus-Pankreasgewebe
• Erfolgreiche IP: Maushautgewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

14941-1-AP bindet in WB, IP, IHC, IF, ELISA BLMH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BLMH fusion protein Ag6888
• Vollständiger Name: bleomycin hydrolase
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC003616
• Gene symbol: BLMH
• Gene ID (NCBI): 642
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BLMH(Bleomycin hydrolase) is also named as BH, BMH and belongs to the peptidase C1 family. The gene is expressed in many human tissues with elevated expression levels found in testis, skeletal muscle, and pancreas, and low levels of expression in colon and peripheral blood leukocytes(PMID:8639621). The protein catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity. BH-immunoreactivity is essentially located in the cytoplasm and at the outer membrane of the rough endoplasmic reticulum of epithelial cells of the kidney, as well as in that of hepatocytes of the liver(PMID:17158865).

Publikationen

Species	Application	Title
hamster	IF	BMC Mol Biol; DNA double-strand break repair is impaired in presenescent Syrian hamster fibroblasts.; Authors - Ljudmila Solovjeva
mouse	IHC	Int J Oncol; N6-methyladenosine-induced long non-coding RNA PVT1 regulates the miR-27b-3p/BLM axis to promote prostate cancer progression; Authors - Bin Chen
human	WB	J Dermatol Sci; Increased production of natural moisturizing factors and bleomycin hydrolase activity in elderly human skin; Authors - Munehiro Tsurumachi