BLNK Monoklonaler Antikörper

BLNK Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1A1A1

Kat-Nr. : CL594-66953

Synonyme

SLP65, Cytoplasmic adapter protein, B-cell linker protein, B-cell adapter containing a Src homology 2 domain protein, B-cell adapter containing a SH2 domain protein

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  IF Staining of NIH/3T3 using CL594-66953

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite®594 BLNK antibody (CL594-66953, Clone: 1A1A1 ) at dilution of 1:200.

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  FC experiment of Daudi using CL594-66953

  1X10^6 Daudi cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human BLNK (CL594-66953, Clone:1A1A1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen
• Erfolgreiche Detektion in FC (Intra): Daudi-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66953 bindet in IF/ICC, FC (Intra) BLNK und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BLNK fusion protein Ag28673
• Vollständiger Name: B-cell linker
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC018906
• Gene symbol: BLNK
• Gene ID (NCBI): 29760
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BLNK (also known as SLP-65 or BASH) is an important adaptor protein selectively expressed in B-lineage cells. BLNK bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. BLNK has been shown to be necessary for BCR-mediated Ca2+ mobilization, for the activation of mitogen-activated protein kinases such as ERK, JNK, and p38 in a chicken B cell line DT40, and for activation of transcription factors such as NF-AT and NF-κB in human or mouse B cells. BLNK plays a crucial role in pre-BCR-dependent progression of B cell development, BCR-mediated B cell survival, activation, proliferation, and T-independent immune responses.