BLVRB Polyklonaler Antikörper

BLVRB Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 17727-1-AP

Synonyme

BVR-B, BVRB, BVR B, Biliverdin-IX beta-reductase, Biliverdin reductase B

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  WB analysis using 17727-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17727-1-AP (BLVRB antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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  WB analysis of HepG2 using 17727-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17727-1-AP (BLVRB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 17727-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 17727-1-AP (BLVRB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 17727-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 17727-1-AP (BLVRB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 17727-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 17727-1-AP (BLVRB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 17727-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17727-1-AP (BLVRB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 17727-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17727-1-AP (BLVRB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 17727-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 17727-1-AP (BLVRB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 17727-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17727-1-AP (BLVRB Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 17727-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17727-1-AP (BLVRB Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, humanes Lebergewebe, L02-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, Rattennierengewebe, Rattenlebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

17727-1-AP bindet in WB, IHC, ELISA BLVRB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BLVRB fusion protein Ag12124
• Vollständiger Name: biliverdin reductase B (flavin reductase (NADPH))
• Berechnetes Molekulargewicht: 206 aa, 22 kDa
• Beobachtetes Molekulargewicht: 22-27 kDa
• GenBank-Zugangsnummer: BC109371
• Gene symbol: BLVRB
• Gene ID (NCBI): 645
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Oxid Med Cell Longev; Bilirubin Oxidation End Products (BOXes) Induce Neuronal Oxidative Stress Involving the Nrf2 Pathway.; Authors - Yinzhong Lu