BLZF1 Polyklonaler Antikörper

BLZF1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 16354-1-AP

Synonyme

GOLGIN 45, Golgin-45, JEM 1, JEM1, JEM-1

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  WB analysis of K-562 using 16354-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16354-1-AP (BLZF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16354-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16354-1-AP (BLZF1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16354-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16354-1-AP (BLZF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 16354-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16354-1-AP (BLZF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16354-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16354-1-AP (BLZF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 16354-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16354-1-AP (BLZF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 16354-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16354-1-AP (BLZF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

16354-1-AP bindet in WB, IHC, ELISA BLZF1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BLZF1 fusion protein Ag9564
• Vollständiger Name: basic leucine zipper nuclear factor 1
• Berechnetes Molekulargewicht: 400 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC020716
• Gene symbol: BLZF1
• Gene ID (NCBI): 8548
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BLZF1 (basic leucine zipper nuclear factor 1), also known as JEM1 or Golgin-45, maps to human chromosome 1q24, encodes for a 3 kb mRNA that is translated into a 45 kDa protein. BLZF1 is a member of b-ZIP family, and has been implicated in epigenetic regulation and Wnt/β-catenin signaling.

Publikationen

Species	Application	Title
human	WB	Mol Biol Cell; Knockout of the Golgi stacking proteins GRASP55 and GRASP65 impairs Golgi structure and function.; Authors - Michael E Bekier
human	WB	Methods Mol Biol; Immunofluorescence Microscopy of the Mammalian Golgi Apparatus; Authors - Maryam Arab
	WB	Methods Mol Biol; Common Markers and Small Molecule Inhibitors in Golgi Studies; Authors - Sarah Bui