BMI1 Monoklonaler Antikörper

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• KD/KO Validated

BMI1 Monoklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Affe, human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

CloneNo.

2E10A11

Kat-Nr. : 66161-1-Ig

Synonyme

BMI1, PCGF4, Polycomb complex protein BMI 1, RING finger protein 51, RNF51

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Galerie der Validierungsdaten

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  WB analysis of PC-12 using 66161-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 66161-1-Ig (BMI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 66161-1-Ig

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 66161-1-Ig (BMI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 66161-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 66161-1-Ig (BMI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 66161-1-Ig

  U-937 cells were subjected to SDS PAGE followed by western blot with 66161-1-Ig (BMI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 66161-1-Ig

  IP Result of anti-BMI1 (IP:66161-1-Ig, 3ug; Detection:66161-1-Ig 1:1000) with HEK-293 cells lysate 1800ug.

• 

  IHC staining of human lymphoma using 66161-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66161-1-Ig (BMI1 Antibody) at dilution of 1:50 under 40x lens.

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  IHC staining of human breast cancer using 66161-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66161-1-Ig (BMI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 66161-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66161-1-Ig (BMI1 Antibody) at dilution of 1:50 under 10x lens.

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  IF Staining of COS-7 using 66161-1-Ig

  Immunofluorescent analysis of COS-7 cells using 66161-1-Ig (BMI1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen, HT-1080.Zellen, U2OS-Zellen, U-937-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: COS-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Produktinformation

66161-1-Ig bindet in WB, IP, IHC, IF, ELISA BMI1 und zeigt Reaktivität mit Affe, human, Maus

• Getestete Reaktivität: Affe, human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BMI1 fusion protein Ag21284
• Vollständiger Name: BMI1 polycomb ring finger oncogene
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 37-43 kDa
• GenBank-Zugangsnummer: BC011652
• Gene symbol: BMI1
• Gene ID (NCBI): 648
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BMI- 1 is one of polycomb group genes, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit. Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16Ink4a and p19Arf genes and is required for maintenance of adult hematopoietic and neural stem cells. The antibody reacts with the 37kd BMI1 protein.

Publikationen

Species	Application	Title
human	WB	Autophagy; Inhibition of BMI1 induces autophagy-mediated necroptosis.; Authors - Anindya Dey ; KD Validated
mouse	WB	Clin Transl Med; Bmi-1-RING1B prevents GATA4-dependent senescence-associated pathological cardiac hypertrophy by promoting autophagic degradation of GATA4.; Authors - Haiyun Chen
mouse	WB	J Bone Miner Res; Bmi-1 Overexpression Improves Sarcopenia Induced by 1,25(OH)2 D3 Deficiency and Downregulates GATA4-Dependent Rela Transcription; Authors - Qiuyi Wang
human	WB	Aging (Albany NY); Identification of a mesenchymal-related signature associated with clinical prognosis in glioma.; Authors - Zhengwei Zhang
human,mouse	WB,IHC	Mol Cancer Ther; Evaluating the Mechanism and Therapeutic Potential of PTC-028, a Novel Inhibitor of BMI-1 Function in Ovarian Cancer.; Authors - Anindya Dey
human	WB,IF	Cancer Sci; Ubiquitin-specific protease 22 acts as an oncoprotein to maintain glioma malignancy through deubiquitinating B cell-specific Moloney murine leukemia virus integration site 1 for stabilization.; Authors - Guan-Zhong Qiu