BMP15 Polyklonaler Antikörper

BMP15 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 18982-1-AP

Synonyme

BMP 15, BMP15, BMP-15, bone morphogenetic protein 15, GDF 9B, GDF9B, ODG2, POF4

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  WB analysis of HeLa using 18982-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18982-1-AP (BMP15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 18982-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18982-1-AP (BMP15 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 18982-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18982-1-AP (BMP15 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 18982-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18982-1-AP (BMP15 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 18982-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18982-1-AP (BMP15 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

18982-1-AP bindet in WB, IHC, IF, CoIP, ELISA BMP15 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: bone morphogenetic protein 15
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: NM_005448
• Gene symbol: BMP15
• Gene ID (NCBI): 9210
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BMP15, also named as GDF9B, is a member of the bone morphogenetic protein family which is part of the transforming growth factor beta superfamily. The transforming growth factor beta superfamily includes large families of growth and differentiation factors. BMP15 may be involved follicular development and oocyte-specific growth/differentiation factor that stimulates folliculogenesis and granulosa cell (GC) growth. Mutation of BMP15 will cause of ovarian dysgenesis type 2 (ODG2) and premature ovarian failure type 4 (POF4).

Publikationen

Species	Application	Title
human	WB,CoIP	Cell Commun Signal; GDF9His209GlnfsTer6/S428T and GDF9Q321X/S428T bi-allelic variants caused female subfertility with defective follicle enlargement; Authors - Yuwei Duan
human	WB	Mol Med Rep; Abnormal expression levels of BMP15/Smad1 are associated with granulosa cell apoptosis in patients with polycystic ovary syndrome.; Authors - Xiangrong Cui
mouse	IHC	Proc Natl Acad Sci U S A; A rare germline BMP15 missense mutation causes hereditary ovarian immature teratoma in human; Authors - Yakun Liu
mouse	WB	Adv Sci (Weinh); AMPK Suppression Due to Obesity Drives Oocyte mtDNA Heteroplasmy via ATF5-POLG Axis; Authors - Yanting Chen
mouse	WB	Environ Toxicol; Acrylamide Exposure Impairs Ovarian Tricarboxylic Acid Cycle and Reduces Oocyte Quality in Mouse; Authors - Yue-Cen Liu
mouse	IF	Reprod Sci; Melatonin Protects Against Mitochondrial Dyshomeostasis and Ovarian Damage Caused by Chronic Unpredictable Mild Stress Through the eIF2α-AFT4 Signaling Pathway in Mice; Authors - Si-Min Ding