BMP3 Polyklonaler Antikörper

BMP3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 22188-1-AP

Synonyme

BMP 3, BMP 3A, BMP3, BMP-3, BMP3A, bone morphogenetic protein 3, Bone morphogenetic protein 3A, Osteogenin

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  WB analysis of Recombinant protein using 22188-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 22188-1-AP (BMP3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 22188-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22188-1-AP (BMP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 22188-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22188-1-AP (BMP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 22188-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22188-1-AP (BMP3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 22188-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22188-1-AP (BMP3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 22188-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22188-1-AP (BMP3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 22188-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22188-1-AP (BMP3 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lebergewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

22188-1-AP bindet in WB, IHC, ELISA BMP3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BMP3 fusion protein Ag16750
• Vollständiger Name: bone morphogenetic protein 3
• Berechnetes Molekulargewicht: 472 aa, 53 kDa
• GenBank-Zugangsnummer: BC096269
• Gene symbol: BMP3
• Gene ID (NCBI): 651
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BMP3 belongs to the transforming growth factor-beta (TGFB) superfamily. BMP3 plays an important role in embryonic development by inducing and patterning early skeletal formation. BMP3 has also been reported as a negative growth regulator in bone marrow progenitor cells through the inhibition of DNA synthesis and proliferation. Additionally, recent studies have revealed that BMP3 contributes to tumor development and progression.

Publikationen

Species	Application	Title
human	WB	Biochem Biophys Res Commun; Bone morphogenetic protein-3 is a negative regulator of transforming growth factor beta and fibrosis; Authors - Emma R Dorris
human	WB	Biol Trace Elem Res; Effects of Sodium Fluoride Treatment In Vitro on Cell Proliferation, BMP-2 and BMP-3 Expression in Human Osteosarcoma MG-63 Cells.; Authors - Yan Wei