BPGM Polyklonaler Antikörper

BPGM Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 17173-1-AP

Synonyme

2,3 bisphosphoglycerate mutase, Bisphosphoglycerate mutase, BPG dependent PGAM, BPGM

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 17173-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 17173-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 17173-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 17173-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 17173-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 17173-1-AP

  IP result of anti-BPGM (IP:17173-1-AP, 4ug; Detection:17173-1-AP 1:700) with mouse liver tissue lysate 6000 ug.

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  IHC staining of human liver cancer using 17173-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17173-1-AP (BPGM Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 17173-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17173-1-AP (BPGM Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 17173-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17173-1-AP (BPGM Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 17173-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17173-1-AP (BPGM Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, Mausherzgewebe, Mauslebergewebe, Mauslungengewebe, Rattenlebergewebe, U-87 MG-Zellen
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

17173-1-AP bindet in WB, IP, IHC, IF, ELISA BPGM und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BPGM fusion protein Ag10731
• Vollständiger Name: 2,3-bisphosphoglycerate mutase
• Berechnetes Molekulargewicht: 259 aa, 30 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC017050
• Gene symbol: BPGM
• Gene ID (NCBI): 669
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human,mouse	WB,IP	Cell Metab; Erythrocyte transglutaminase-2 combats hypoxia and chronic kidney disease by promoting oxygen delivery and carnitine homeostasis.; Authors - Ping Xu
rat	WB,IF	Brain Res Bull; Enhanced BPGM/2,3-DPG pathway activity suppresses glycolysis in hypoxic astrocytes via FIH-1 and TET2; Authors - Guoji E