BZW1 Polyklonaler Antikörper

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• KD/KO Validated

BZW1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 19917-1-AP

Synonyme

5MP2, Basic leucine zipper and W2 domain-containing protein 1, BZAP45, eIF5-mimic protein 2, KIAA0005

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  WB analysis of MCF-7 using 19917-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19917-1-AP (BZW1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19917-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19917-1-AP (BZW1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 19917-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19917-1-AP (BZW1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 19917-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19917-1-AP (BZW1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung cancer using 19917-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 19917-1-AP (BZW1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 19917-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 19917-1-AP (BZW1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 19917-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using BZW1 antibody (19917-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of A431 using 19917-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using BZW1 antibody (19917-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

19917-1-AP bindet in WB, IHC, IF/ICC, ELISA BZW1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BZW1 fusion protein Ag13830
• Vollständiger Name: basic leucine zipper and W2 domains 1
• Berechnetes Molekulargewicht: 353 aa, 41 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC001804
• Gene symbol: BZW1
• Gene ID (NCBI): 9689
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BZW1, also known as basic leucine zipper and W2 domains 1, is a member of the basic leucine zipper (bZIP) superfamily of transcription factors. It is a 45 kDa protein that contains an N-terminal bZIP domain for protein interactions and a C-terminal nucleotide (ATP or GTP) binding domain. Human BZW1 can activate transcription of the histone H4 gene and serve as a co-regulator with other transcription factors to control the cell cycle. In recent years, BZW1 has been identified as enhancing phosphorylation to promote glycolysis in pancreatic ductal adenocarcinoma. Moreover, BZW1 has been found to regulate the cell cycle in ovarian cancer, thereby promoting its progression. Additionally, BZW1 plays a crucial role in mucoepidermoid carcinoma of the salivary glands. BZW1 is also involved in the regulation of translation initiation, acting as a translational rheostat and autoregulating its own translation. It has been suggested that BZW1, as well as its paralog BZW2, is an eIF5-mimic protein. BZW1 has been shown to facilitate glycolysis and promote tumor growth in pancreatic ductal adenocarcinoma through potentiating eIF2α phosphorylation, and it may serve as a therapeutic target for patients with pancreatic cancer. In macrophages, activation of BZW1 by CEBPB promotes eIF2α phosphorylation-mediated metabolic reprogramming and endoplasmic reticulum stress. BZW1 has also been found to be associated with the Wnt/β-catenin pathway in lung adenocarcinoma, potentially influencing epithelial-mesenchymal transition (EMT) processes.

Publikationen

Species	Application	Title
human	WB,IF	Leukemia; IPO11 regulates the nuclear import of BZW1/2 and is necessary for AML cells and stem cells.; Authors - Boaz Nachmias
human,mouse	WB,IHC,IF	Genomics; BZW1 as an oncogene is associated with patient prognosis and the immune microenvironment in glioma; Authors - Linyao Zhao ; KD Validated