ATG14/Barkor (C-terminal) Polyklonaler Antikörper

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• KD/KO Validated

ATG14/Barkor (C-terminal) Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 24412-1-AP

Synonyme

ATG14, Atg14L, Barkor, KIAA0831

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 24412-1-AP

  WB result of ATG14/Barkor (C-terminal) antibody (24412-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG14/Barkor (C-terminal) transfected HeLa cells.

• 

  WB analysis of mouse brain using 24412-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24412-1-AP (ATG14/Barkor (C-terminal) antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 24412-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 24412-1-AP (ATG14/Barkor (C-terminal) antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 24412-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 24412-1-AP (ATG14/Barkor (C-terminal) antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 24412-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 24412-1-AP (ATG14/Barkor (C-terminal) antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 24412-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 24412-1-AP (ATG14/Barkor (C-terminal) antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 24412-1-AP

  Immunofluorescent analysis of HeLa cells using 24412-1-AP (ATG14/Barkor (C-terminal) antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 2 publications below

Produktinformation

24412-1-AP bindet in WB, IHC, IF, ELISA ATG14/Barkor (C-terminal) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATG14/Barkor (C-terminal) fusion protein Ag19707
• Vollständiger Name: KIAA0831
• Berechnetes Molekulargewicht: 492 aa, 55 kDa
• Beobachtetes Molekulargewicht: 65-68 kDa, 42 kDa
• GenBank-Zugangsnummer: BC109090
• Gene symbol: KIAA0831
• Gene ID (NCBI): 22863
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Barkor, also named as ATG14, Atg14L and KIAA0831, is named as Beclin 1-associated autophagy-related key regulator protein. The function of Barkor in autophagy has been manifested in several assays, including stress-induced LC3 lipidation, autophagosome formation, and Salmonella typhimurium amplification. A major band around 65-68 kDa and a light band around 42 kDa can be observed in western blot which may be caused by alternative splicing of ATG14. The specificity of ATG14 antibody has been tested by siRNA test.

Publikationen

Species	Application	Title
mouse	WB	Cell Death Differ; ATG4D is the main ATG8 delipidating enzyme in mammalian cells and protects against cerebellar neurodegeneration.; Authors - Isaac Tamargo-Gómez
human	IF	J Cell Sci; Syntaxin 17, an ancient SNARE paralog, plays different and conserved roles in different organisms.; Authors - Shun Kato
human	WB	Microb Pathog; Roles of bovine viral diarrhea virus envelope glycoproteins in inducing autophagy in MDBK cells.; Authors - Qiang Fu
mouse	WB,IF	Exp Eye Res; Inhibiting miR-129-5p alleviates inflammation and modulates autophagy by targeting ATG14 in fungal keratitis.; Authors - Jingqiong Lin
human	WB	EMBO Rep; An ULK1/2-PXN mechanotransduction pathway suppresses breast cancer cell migration; Authors - Peigang Liang