BCL2L1 Polyklonaler Antikörper

BCL2L1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(77)

Kat-Nr. : 26967-1-AP

Synonyme

Bcl-xL, Apoptosis regulator Bcl X, Apoptosis regulator Bcl-X, Bcl2-L-1, Bcl-2-like protein 1

---

• 

  WB analysis of NIH/3T3 using 26967-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26967-1-AP (Bcl-XL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 26967-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26967-1-AP (Bcl-XL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of NIH/3T3 using 26967-1-AP

  IP result of anti-Bcl-XL (IP:26967-1-AP, 4ug; Detection:26967-1-AP 1:1000) with NIH/3T3 cells lysate 2000 ug.

• 

  IHC staining of human testis using 26967-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26967-1-AP (Bcl-XL antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 26967-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26967-1-AP (Bcl-XL antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung cancer using 26967-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26967-1-AP (Bcl-XL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 26967-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26967-1-AP (Bcl-XL/S antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 26967-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Bcl-XL antibody (26967-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of Jurkat using 26967-1-AP

  1x10^6 200 Jurkat cells were intracellularly stained with 0.4 ug Bcl-XL Polyclonal antibody (26967-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, RAW 264.7-Zellen
• Erfolgreiche IP: NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): 200 Jurkat cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 75 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Produktinformation

26967-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA BCL2L1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BCL2L1 fusion protein Ag25604
• Vollständiger Name: BCL2-like 1
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC019307
• Gene symbol: BCL2L1
• Gene ID (NCBI): 598
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. This antibody can recognize Bcl-XL, Bcl-XS and Bcl-X(Beta).

Publikationen

Species	Application	Title
human	WB	Adv Sci (Weinh); Mitochondrial tRNAGlu 14693A>G Mutation, an "Enhancer" to the Phenotypic Expression of Leber's Hereditary Optic Neuropathy; Authors - Lihao Jin
rat	WB	Microbiome; The microbiota-gut-brain axis participates in chronic cerebral hypoperfusion by disrupting the metabolism of short-chain fatty acids.; Authors - Weiping Xiao
human	WB	Clin Cancer Res; Long noncoding RNA LINC01234 functions as a competing endogenous RNA to regulate CBFB expression by sponging miR-204-5p in gastric cancer.; Authors - Xin Chen
human	WB	Clin Cancer Res; Inhibition of Bcl-2 Synergistically Enhances the Antileukemic Activity of Midostaurin and Gilteritinib in Preclinical Models of FLT3-mutated Acute Myeloid Leukemia.; Authors - Jun Ma
human	WB	Cell Rep; Developmental potency of human ES cell-derived mesenchymal stem cells revealed in mouse embryos following blastocyst injection; Authors - Borong Huang
mouse	WB	Int J Biol Macromol; CircDYM attenuates microglial apoptosis via CEBPB/ZC3H4 axis in LPS-induced mouse model of depression; Authors - Zhongqiu Zhou