Bcl2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Bcl2 Polyklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte und mehr (7)

Anwendung

WB, IHC, IF, FC (Intra), CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(1117)

Rezensionen (3)

Kat-Nr. : 26593-1-AP

Synonyme

Bcl-2, B cell leukemia/lymphoma 2, Bcl 2

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  WB analysis using 26593-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26593-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26593-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26593-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26593-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 26593-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 26593-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C2C12 using 26593-1-AP

  C2C12 cells were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse spleen using 26593-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 26593-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 26593-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 26593-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 26593-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of NIH/3T3 using 26593-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Bcl2 antibody (26593-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of NIH/3T3 using 26593-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 26593-1-AP (Bcl2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of NIH/3T3 using 26593-1-AP

  1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug Bcl2 Polyclonal antibody (26593-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, C2C12-Zellen, Maushirngewebe, Maus-Kolongewebe, Mausherzgewebe, Mausnierengewebe, Mausmilzgewebe, Maushodengewebe, Rattennierengewebe, Rattenmilzgewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: Mausmilzgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 1067 publications below
• IHC: See 62 publications below
• IF: See 23 publications below
• CoIP: See 1 publications below

Produktinformation

26593-1-AP bindet in WB, IHC, IF, FC (Intra), CoIP, ELISA Bcl2 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• In Publikationen genannte Reaktivität: Ente, hamster, Hausschwein, Huhn, Hund, Kaninchen, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Bcl2 fusion protein Ag24260
• Vollständiger Name: B-cell leukemia/lymphoma 2
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: NM-009741
• Gene symbol: Bcl2
• Gene ID (NCBI): 12043
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

1.       What is Bcl-2?

Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.

2.       FAQs for Bcl-2

1.  How to measure Bcl-2 and Bax apoptotic markers by western blotting

   Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.
   

2. What band size should I expect in western blotting for Bcl-2?

   The molecular weight of Bcl-2 is 26 kDa.

Publikationen

Species	Application	Title
mouse	WB	ACS Nano; Dual Strategies Based on Golgi Apparatus/Endoplasmic Reticulum Targeting and Anchoring for High-Efficiency siRNA Delivery and Tumor RNAi Therapy; Authors - Yashi Wang ; KD Validated
		ACS Nano; Melatonin-Derived Carbon Dots with Free Radical Scavenging Property for Effective Periodontitis Treatment via the Nrf2/HO-1 Pathway; Authors - Xirui Xin
mouse	WB	Acta Pharm Sin B; Honokiol alleviated neurodegeneration by reducing oxidative stress and improving mitochondrial function in mutant SOD1 cellular and mouse models of amyotrophic lateral sclerosis; Authors - Yujun Zhou
		Cell Rep Med; Microneedle delivery of CAR-M-like engineered macrophages alleviates intervertebral disc degeneration through enhanced efferocytosis capacity; Authors - Xingyu Zhou
mouse	WB	Redox Biol; FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response; Authors - Zhe Wang
mouse	WB	Redox Biol; Protective role of vitamin D receptor against mitochondrial calcium overload from PM2.5-Induced injury in renal tubular cells; Authors - Mengqiu Lu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Charlotte (Verified Customer) (08-15-2024)

The fainter band at the bottom of the membrane corresponds to the expected MW. Done in milk

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: NIH-3T3

Bcl2 Antibody Western Blot validation (1/1000 dilution) in NIH-3T3 (Cat no:26593-1-AP)

FH

Anna (Verified Customer) (10-04-2022)

Promising staining. I have a problem with attaching a photo file. Please check the file below. https://drive.google.com/file/d/15d3t6thx7rcWyN-_DkkKCtpAMyi8jxHO/view

• Applications: Immunofluorescence
• Primary Antibody Dilution: 500
• Cell Tissue Type: brain frozen sections - mouse

FH

PRADEEP (Verified Customer) (11-03-2020)

Giving very good result for IF.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse muscle