Beta Catenin Rekombinanter Antikörper

Beta Catenin Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte, Zebrafisch

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

2J24

Kat-Nr. : 80488-1-RR

Synonyme

2J24, b cat, B-catenin, beta-Catenin, Catenin beta 1

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  WB analysis using 80488-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80488-1-RR (Beta Catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of zebrafish using 80488-1-RR

  zebrafish tissue was subjected to SDS PAGE followed by western blot with 80488-1-RR (Beta Catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 80488-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 80488-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human rectal cancer using 80488-1-RR

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human rectal cancer using 80488-1-RR

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 80488-1-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 80488-1-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 80488-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 80488-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 80488-1-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (80488-1-RR, Clone: 2J24 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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  ChIP experiment of HCT 116 using 80488-1-RR

  Chromatin was prepared from HCT 116 cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of β-Catenin (80488-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, LNCaP-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, Hausschwein-Hirngewebe, Zebrafischgewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen
• Erfolgreiche Detektion in ChIP-qPCR: HCT 116-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:1000-1:4000
• CHImmunpräzipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

80488-1-RR bindet in WB, IHC, IF/ICC, CoIP, ELISA, ChIP-qPCR Beta Catenin und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten, Zebrafisch

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte, Zebrafisch
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: catenin (cadherin-associated protein), beta 1, 88kDa
• Berechnetes Molekulargewicht: 781 aa, 86 kDa
• Beobachtetes Molekulargewicht: 92 kDa
• GenBank-Zugangsnummer: BC058926
• Gene symbol: Beta Catenin
• Gene ID (NCBI): 1499
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)

Publikationen

Species	Application	Title
human	WB,IF,CoIP	Cell Biol Toxicol; M6A modification-mediated LIMA1 promotes the progression of hepatocellular carcinoma through the wnt-βcatenin/Hippo pathway; Authors - Chao Zhang
human	WB	Discov Oncol; miR-124-3p inhibits CRC proliferation, migration, and invasion by targeting ITGB1; Authors - Jing Li