Biglycan Monoklonaler Antikörper

Biglycan Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1E3F2

Kat-Nr. : 67275-1-Ig

Synonyme

BGN, biglycan, Bone/cartilage proteoglycan I, DSPG1, PG S1, PGI, SLRR1A

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Galerie der Validierungsdaten

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  WB analysis of pig skin using 67275-1-Ig

  pig skin tissue were subjected to SDS PAGE followed by western blot with 67275-1-Ig (Biglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 67275-1-Ig

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 67275-1-Ig (Biglycan antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67275-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67275-1-Ig (Biglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig colon using 67275-1-Ig

  pig colon tissue were subjected to SDS PAGE followed by western blot with 67275-1-Ig (Biglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung using 67275-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 67275-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67275-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67275-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67275-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67275-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 67275-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 67275-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 67275-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 67275-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 67275-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 67275-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 67275-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 67275-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Hautgewebe, Hausschwein-Kolongewebe, HepG2-Zellen, Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Kolonkarzinomgewebe, humanes Lebergewebe, humanes Lungengewebe, humanes Lungenkarzinomgewebe, humanes Magenkrebsgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

67275-1-Ig bindet in WB, IHC, ELISA Biglycan und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Biglycan fusion protein Ag8012
• Vollständiger Name: biglycan
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC004244
• Gene symbol: BGN
• Gene ID (NCBI): 633
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Biglycan is a member of the small leucine-rich proteoglycan (SLRP) family (PMID: 18463092). It consists of a core protein of 42 kDa containing leucine-rich repeats (LRRs), to which two chondroitin/dermatan sulfate side chains are covalently bound (PMID: 2212616; 10383378). The tissue-specific chondroitin- or dermatan-sulfate glycosaminoglycan chains of biglycan are attached to amino acid residues at the N-terminus of the core protein (PMID: 22821552). Biglycan is a ubiquitous component of connective tissue matrix and may act as a signaling molecule (PMID: 22821552). Upregulation of biglycan has been reported in multiple types of solid cancer (PMID: 32194659).

Publikationen

Species	Application	Title
human	WB	iScience; Biglycan regulated colorectal cancer progress by modulating enteric neuron-derived IL-10 and abundance of Bacteroides thetaiotaomicron; Authors - Yuyu Xu