EDRF1 Polyklonaler Antikörper

EDRF1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 21883-1-AP

Synonyme

C10orf137, Erythroid differentiation-related factor 1, RP11 383C5.6

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  WB analysis using 21883-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 21883-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 21883-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21883-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21883-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21883-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 21883-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 21883-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21883-1-AP (EDRF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 21883-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21883-1-AP (EDRF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 21883-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21883-1-AP (EDRF1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 21883-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 21883-1-AP (EDRF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 21883-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 21883-1-AP (EDRF1 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, humanes Hirngewebe, MCF-7-Zellen, Maushirngewebe, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

21883-1-AP bindet in WB, IHC, ELISA EDRF1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EDRF1 fusion protein Ag16441
• Vollständiger Name: chromosome 10 open reading frame 137
• Berechnetes Molekulargewicht: 1238 aa, 139 kDa
• Beobachtetes Molekulargewicht: 140-160 kDa
• GenBank-Zugangsnummer: BC107479
• Gene symbol: EDRF1
• Gene ID (NCBI): 26098
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EDRF1, also named as C10orf137, is a transcription factor involved in erythroid differentiation. It regulates alpha- and gamma-globin gene synthesis by modulating DNA-binding activity of GATA-1 transcription factor. EDRF1 has four isoforms with MW 66 kDa, 89 kDa and 135-140 kDa.

Publikationen

Species	Application	Title
human	WB	iScience; Differential localization of dengue virus protease affects cell homeostasis and triggers to thrombocytopenia; Authors - Lekha Gandhi