Hikeshi Polyklonaler Antikörper

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• KD/KO Validated

Hikeshi Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 20524-1-AP

Synonyme

C11orf73, HSPC138, HSPC179, HSPC248, L7RN6

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  WB analysis of PC-3 using 20524-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20524-1-AP (Hikeshi antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 20524-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 20524-1-AP (Hikeshi antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20524-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20524-1-AP (Hikeshi antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 20524-1-AP

  IP result of anti-Hikeshi (IP:20524-1-AP, 4ug; Detection:20524-1-AP 1:500) with mouse brain tissue lysate 3000ug.

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  IHC staining of human lung using 20524-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 20524-1-AP (Hikeshi antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 20524-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 20524-1-AP (Hikeshi antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of A375 using 20524-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using Hikeshi antibody (20524-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, A375-Zellen, Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A375-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

20524-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA Hikeshi und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Hikeshi fusion protein Ag14376
• Vollständiger Name: chromosome 11 open reading frame 73
• Berechnetes Molekulargewicht: 197 aa, 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC006476
• Gene symbol: Hikeshi
• Gene ID (NCBI): 51501
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat,mouse	WB,IHC,IF	Dev Cell; Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination; Authors - Li Li ; KO Validated
human	WB	Life Sci Alliance; Lack of Hikeshi activates HSF1 activity under normal conditions and disturbs the heat-shock response.; Authors - Shingo Kose
mouse	IF	Medicines (Basel); The Infantile Leukoencephalopathy-Associated Mutation of C11ORF73/HIKESHI Proteins Generates de novo Interactive Activity with Filamin A, Inhibiting Oligodendroglial Cell Morphological Differentiation.; Authors - Kohei Hattori
human	WB,IF	Am J Med Genet A; A novel missense variant in HIKESHI: Clinical phenotype, in vitro functional testing, and potential for gene therapy; Authors - Eric J Mallack