C13orf18 Polyklonaler Antikörper

C13orf18 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 21183-1-AP

Synonyme

Pacer, Protein associated with UVRAG as autophagy enhancer, Protein Rubicon-like, RUBCNL

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  WB analysis of human skeletal muscle using 21183-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21183-1-AP (C13orf18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 21183-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21183-1-AP (C13orf18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 21183-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21183-1-AP (C13orf18 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 21183-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21183-1-AP (C13orf18 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 21183-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21183-1-AP (C13orf18 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 21183-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21183-1-AP (C13orf18 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 21183-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 21183-1-AP (C13orf18 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 21183-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 21183-1-AP (C13orf18 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of U-251 using 21183-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using C13orf18 antibody (21183-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Skelettmuskelgewebe, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

21183-1-AP bindet in WB, IHC, IF/ICC, ELISA C13orf18 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C13orf18 fusion protein Ag15474
• Vollständiger Name: chromosome 13 open reading frame 18
• Berechnetes Molekulargewicht: 662 aa, 73 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC032311
• Gene symbol: C13orf18
• Gene ID (NCBI): 80183
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nature; Architecture of the human interactome defines protein communities and disease networks.; Authors - Edward L Huttlin
human	WB	Precis Clin Med; Blockade of Syk modulates neutrophil immune-responses via the mTOR/RUBCNL-dependent autophagy pathway to alleviate intestinal inflammation in ulcerative colitis; Authors - Fengqin Zhu