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  • Featured Product
  • KD/KO Validated

CTC1 Polyklonaler Antikörper

CTC1 Polyklonal Antikörper für WB, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF-P, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28268-1-AP

Synonyme

C17orf68, AAF 132, CST complex subunit CTC1, HBV DNAPTP1-transactivated protein B

Filter:
  • Western Blot (WB) analysis of various lysates using CTC1 Polyclonal antibody (28268-1-AP)

    WB analysis using 28268-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28268-1-AP (C17orf68 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CTC1 Polyclonal antibody (28268-1-AP)

    WB analysis of HeLa using 28268-1-AP

    WB result of C17orf68 antibody (28268-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C17orf68 transfected HeLa cells.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CTC1 Polyclonal antibody (28268-1-AP)

    IF Staining of mouse brain using 28268-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CTC1 antibody (28268-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBA549-Zellen, HeLa-Zellen, K562-Zellen
Erfolgreiche Detektion in IF-PMaushirngewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:3000
Immunfluoreszenz (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

28268-1-AP bindet in WB, IF-P, ELISA CTC1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CTC1 fusion protein Ag28167
Vollständiger Name chromosome 17 open reading frame 68
Berechnetes Molekulargewicht 1217 aa, 135 kDa
Beobachtetes Molekulargewicht 135-150 kDa
GenBank-ZugangsnummerBC111783
Gene symbol CTC1
Gene ID (NCBI) 80169
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Conserved telomere maintenance component 1 (CTC1) is a subunit of an alpha accessory factor (AAF) that stimulates the activity of DNA polymerase-alpha-primase, the enzyme that initiates DNA replication (PMID: 19119139). CTC1 also functions in a telomere-associated complex with STN1 and TEN1 (PMID: 19854130).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for CTC1 antibody 28268-1-APProtokoll herunterladen
IF protocol for CTC1 antibody 28268-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Daniel (Verified Customer) (02-24-2025)

Antibody doesn't specifically recognize CTC1. A band can still be detected in CTC1 knockdown cells.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: RPE1