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NCBP3 Polyklonaler Antikörper

NCBP3 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 24384-1-AP

Synonyme

C17orf85, ELG, HSA277841, Nuclear cap-binding protein subunit 3, Protein ELG

Filter:
  • Western Blot (WB) analysis of various lysates using NCBP3 Polyclonal antibody (24384-1-AP)

    WB analysis using 24384-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24384-1-AP (NCBP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NCBP3 Polyclonal antibody (24384-1-AP)

    IF Staining of HeLa using 24384-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NCBP3 antibody (24384-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBA549-Zellen, HeLa-Zellen, Jurkat-Zellen, PC-3-Zellen
Erfolgreiche Detektion in IF/ICCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 1 publications below

Produktinformation

24384-1-AP bindet in WB, IF/ICC, ELISA NCBP3 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen NCBP3 fusion protein Ag20021
Vollständiger Name chromosome 17 open reading frame 85
Berechnetes Molekulargewicht 620 aa, 70 kDa
Beobachtetes Molekulargewicht 71-80 kDa
GenBank-ZugangsnummerBC148856
Gene symbol NCBP3/C17orf85
Gene ID (NCBI) 55421
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

chromosome 17 open reading frame 85(C17orf85)also named as Protein ELG is 620 amino acids protein, which is localizes in human chromosome 17. The function of protein is still non-known and it consists of some phosphorylation site . It may involves in human embryonic stem cell differentiation.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for NCBP3 antibody 24384-1-APProtokoll herunterladen
IF protocol for NCBP3 antibody 24384-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

RNA Biol

NCBP3/SNHG6 inhibits GBX2 transcription in a histone modification manner to facilitate the malignant biological behaviour of glioma cells.

Authors - Xiwen Li
View Article

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Hana (Verified Customer) (08-20-2019)

Please make more concentrated Ab.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 5% milk
  • Cell Tissue Type: C2C12 myoblast