Draxin Polyklonaler Antikörper

Draxin Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 26342-1-AP

Synonyme

C1orf187, AGPA3119, Dorsal inhibitory axon guidance protein, Neucrin, PSEC0258

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  WB analysis of mouse cerebellum using 26342-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 26342-1-AP (C1orf187 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26342-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 26342-1-AP (C1orf187 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26342-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26342-1-AP (C1orf187 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Cerebellum-Gewebe, Ratten-Cerebellum-Gewebe, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

26342-1-AP bindet in WB, ELISA Draxin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Draxin fusion protein Ag23666
• Vollständiger Name: chromosome 1 open reading frame 187
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC021286
• Gene symbol: C1orf187
• Gene ID (NCBI): 374946
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C1orf187, also named Draxin(Dorsal repulsive axon guidance protein), is a repulsive axon guidance cue. It is widely expressed in the developing hippocampus, glioma, and all histological types of the lung cancer cell. Draxin was shown to bind a 22-amino-acid peptide of the EGF domain of Netrin-1, and through Draxin/Netrin-1 interaction, Draxin may act as a secreted Netrin-1 antagonist. C1orf187 can inhibit or repel neurite outgrowth from dorsal spinal cord. It inhibits the stabilization of cytosolic beta-catenin (CTNNB1) via its interaction with LRP6, thereby acting as an antagonist of Wnt signaling pathway (PMID: 29622850).

Publikationen

Species	Application	Title
human	WB	Front Immunol; Identification of a novel cuproptosis-related gene signature and integrative analyses in patients with lower-grade gliomas; Authors - Jia-hao Bao
human	WB	Chem Biol Interact; Transcriptomic and proteomic study of cancer cell lines exposed to actinomycin D and nutlin-3a reveals numerous, novel candidates for p53-regulated genes; Authors - Barbara Łasut-Szyszka