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C1qC Monoklonaler Antikörper

C1qC Monoklonal Antikörper für IF/ICC

Wirt / Isotyp

Maus / IgM

Getestete Reaktivität

human

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C5B4

Kat-Nr. : CL488-66268

Synonyme

C1Q C, C1QC, C1QG, FLJ27103

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using CoraLite® Plus 488-conjugated C1qC Monoclonal anti (CL488-66268)

    IF Staining of HUVEC using CL488-66268

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using CoraLite® Plus 488 C1qC antibody (CL488-66268, Clone: 1C5B4 ) at dilution of 1:200.

Geprüfte Anwendungen

Erfolgreiche Detektion in IF/ICCHUVEC-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66268 bindet in IF/ICC C1qC und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgM
Klonalität Monoklonal
Typ Antikörper
Immunogen C1qC fusion protein Ag10233
Vollständiger Name complement component 1, q subcomponent, C chain
Berechnetes Molekulargewicht 245 aa, 26 kDa
Beobachtetes Molekulargewicht 26 kDa
GenBank-ZugangsnummerBC009016
Gene symbol C1QC
Gene ID (NCBI) 714
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Thiophiler Affinitätschromatograph
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qC which is the C-chain polypeptide of human complement subcomponent C1q.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IF protocol for CL Plus 488 C1qC antibody CL488-66268Protokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen